Fig 1: Comparison of transcriptomes of invasive and noninvasive organoids reveals differentially expressed genes that are enhanced in invasive protrusions.(A) A sample-to-sample distance plot (Spearman’s correlation) of the variance-stabilizing transform (VST) gene expression for each sample (n = 23). (B) Differentially expressed (DE) genes between all invasive organoids (n = 12) and noninvasive organoids (n = 11) (adjusted-P threshold of 0.05). OLR1, SPOCK1, MMP8, and COL7A1 were all upregulated in invasive organoids compared with the noninvasive organoids. (C) SPOCK1 mRNA expression in indicated organoid groups. Each dot represents an organoid culture. In the box-and-whisker plot, the box boundaries represent the 25th to 75th percentiles, horizontal lines represent medians, and whiskers extend to 1.5 × IQR. (D) Phase and immunofluorescence images of pan-CK, SPOCK1, and DAPI staining in invasive and noninvasive organoids. Scale bars: 50 µm. (E) Immunofluorescence images of pan-CK, SPOCK1, and DAPI staining in primary PDAC tissue sections. Scale bar: 50 µm.
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