Fig 1: Analysis of autophagy regulators. Western blot analysis and immunohistochemical staining on WT, Pfn4+/- and Pfn4-/- mice (n=3/genotype). (A) Western blot using SPECC1L, ARF3 and FKBP1 antibodies on protein extracts from WT, Pfn4+/- and Pfn4-/- testes. (B) Western blot using AKT, AKT1, PI3K, p-PI3K, mTOR, p-mTOR and p-AMPK antibodies on protein extracts from WT, Pfn4+/- and Pfn4-/- testes. (C) Western blot using LC3I/II and SQSTM1 antibodies on protein extracts from WT, Pfn4+/- and Pfn4-/- testes. (D-G) IHC using LC3I/II (D), SQSTM1 (E), AKT1 (F) and ARF3 (G) antibodies on WT, Pfn4+/- and Pfn4-/- testes sections. Scale bars: 50 µm.