Fig 2: Spastin depletion causes polarised distribution of BMPRII. a–d Wild-type HeLa cells (a, c) or HeLa cells staby expressing myc-BMPRII (b, d) were mock-transfected (a, b) or subject to spastin KD by siRNA transfection (c, d), then labelled for the proteins indicated. The panels in the second and fourth rows show higher magnification views of the boxed regions in the panels above and arrows show regions of colocalisation. e HeLa cells stably expressing myc-BMPRII were subject to spastin KD by siRNA transfection, then labelled for the proteins indicated. The lower panels are higher magnification views of the boxed protrusion region in the upper panels. Arrows indicate regions of co-localisation. f Colocalisation between BMPRII versus KIF5 or acetylated tubulin was measured in HeLa cells stably expressing myc-BMPRII and treated with mock siRNA transfection, or spastin siRNA transfection. At least 13 cells quantified in each condition. P values generated by unpaired t tests. Scale bars = 20 μm