Fig 2: Enzymatic deglycosylation assay of AMPA receptor subunits.(A) Representative immunoblots showing changes in electrophoretic mobility for GluA2 and GluA4, but not GluA1 or GluA3, following deglycosylation with Endo H or PNGase F in rat, tree shrew, macaque, and human frontal cortex. (B) Quantification of Endo H-sensitive molecular mass shifts for GluA2 and GluA4. Data are presented as the distance between the Endo H-deglycosylated protein band of higher mobility, and the same protein without Endo H treatment. (C) Quantification of Endo H-sensitive molecular mass shifts for GluA2 and GluA4. Graphs show the ratio of measured intensities of the Endo H-sensitive band to the Endo H-insensitive band. (D) Quantification of PNGase F-sensitive molecular mass shifts for GluA2 and GluA4. Data are presented as the distance between the PNGase F deglycosylated protein band of higher mobility, and the same protein without PNGase F treatment. Graphs represent means ± SD calculated from three independent experiments.