Anti-Mouse IgG (H+L), AP Conjugate S3721 from Promega

Product Specs
ItemAnti-Mouse IgG (H+L), AP Conjugate
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS3721
Quantity100microl
Conjugate/TagAlkaline Phosphatase
ReactivityMouse
TargetIgG (H+L)
SpeciesMouse

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparisonJournal of Genetic Engineering and BiotechnologyMay 22, 2023Nuruliawaty Utami, Dini Nurdiani, Hariyatun Hariyatun, Eko Wahyu Putro, Fadillah Putri Patria, Wien Kusharyotoovernight with shaking at 4 °C. The secondary antibody Anti-mouse IgG (H + L) AP Conjugate, Promega #S3721 (Promega Corporation, Madison, WI, USA) and TBST (a mixture of tris-buffered saline (TBS) and Tween 20

(2) Immunophenotyping of Monocyte Migration Markers and Therapeutic Effects of Selenium on IL-6 and IL-1β Cytokine Axes of Blood Mononuclear Cells in Preoperative and Postoperative Coronary Artery Disease PatientsInternational Journal of Molecular SciencesApril 13, 2023Max Wacker, Anna Ball, Hans-Dietmar Beer, Ingo Schmitz, Katrin Borucki, Faranak Azizzadeh, Maximilian Scherner, George Awad, Jens Wippermann, Priya Veluswamyanti-rabbit IgG, HRP-linked antibody (Cat# 7074, Cell Signaling), and AP-conjugated anti-mouse IgG (S3721, Promega, Madison, WI, USA,) were used as secondary antibodies. As substrate, we used PierceTM ECL Western,

(3) PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosisCell Structure and FunctionDecember 15, 2022Kajung Ryu, Atsushi Yoshida, Yosuke Funato, Daisuke Yamazaki, Hiroaki Mikiperoxidase-conjugated anti-sheep IgG (H+L) (31480) from Pierce; alkaline phosphatase-conjugated anti-mouse IgG (S3721) and anti-rabbit IgG (S3731) from Promega for immunoblotting analysis. We used the following commercially

(4) SIG-1451, a Novel, Non-Steroidal Anti-Inflammatory Compound, Attenuates Light-Induced Photoreceptor Degeneration by Affecting the Inflammatory ProcessInternational Journal of Molecular SciencesAugust 8, 2022Yuki Kikuchi et al.,Santa Cruz Biotechnology (sc-25778) Anti-rabbit IgG,goat,1/7500,Promega (S3731) Anti-mouse IgG,goat,1/7500,Promega (S3721) Antibodies lists.

(5) Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?VirusesFebruary 16, 2022Nakano K, Yokoyama K, Shin S, Uchida K, Tsuji K, Tanaka M, Uchimaru K, Watanabe T.KgaA., Darmstadt, Germany). Secondary antibody: AP (alkaline phosphatase)-conjugated anti-mouse IgG (#S3721), AP-conjugated anti-goat IgG (#V-1151) (both from Promega, Corp., Madison, WI, USA). 2.4.3. NONO Knockdown In

(6) Elucidation of the Mechanism of Host NMD Suppression by HTLV-1 Rex: Dissection of Rex to Identify the NMD Inhibitory DomainVirusesFebruary 9, 2022Kazumi Nakano, Nobuaki Karasawa, Masaaki Hashizume, Yuetsu Tanaka, Takeo Ohsugi, Kaoru Uchimaru, Toshiki WatanabeUniversity of Ryukyus. Secondary antibodies: Alkaline phosphatase (AP)-conjugated anti-mouse IgG (#S3721), AP-conjugated anti-rabbit IgG (#S3731), AP-conjugated anti-rat IgG (#S383A), AP-conjugated anti-goat

(7) Chronological genome and single-cell transcriptome integration characterizes the evolutionary process of adult T cell leukemia-lymphomaNature CommunicationsDecember 1, 2021Makoto Yamagishi et al.Santa Cruz), and β-actin (sc-69879, 1:1000, Santa Cruz). Alkaline phosphatase-conjugated anti-mouse (S3721, 1:2000, Promega) and anti-rabbit (S3731, 1:2000, Promega) secondary antibodies and BCIP/NBT substrate

(8) Contrasting functions of the epithelial‑stromal interaction 1 gene, in human oral and lung squamous cell cancersOncology ReportsNovember 2, 2021Mengmeng Fan, Makoto Arai, Akinobu Tawada, Tetsuhiro Chiba, Reo Fukushima, Katsuhiro Uzawa, Masashi Shiiba, Naoya Kato, Hideki Tanzawa, Yuichi Takiguchi(15 min for each wash), followed by incubation with a secondary antibody with anti-mouse (cat. no. S3721) or anti-rabbit IgG (cat. no. S3731) (1:2,000; Promega Corporation) for 1.5 h at room temperature. Finally,

(9) Molecular and Functional Characterization of MobK Protein—A Novel-Type Relaxase Involved in Mobilization for Conjugational Transfer of Klebsiella pneumoniae Plasmid pIGRKInternational Journal of Molecular SciencesMay 13, 2021Katarzyna Paulina Nowak, Agnieszka Sobolewska-Ruta, Agata Jagiełło, Anna Bierczyńska-Krzysik, Piotr Kierył, Paweł Wawrzyniakmembrane was then incubated with 1:10,000 diluted anti-mouse IgG (H-L) AP-conjugated antibody (Promega, S3721, Madison, WI, USA) for 1 h at room temperature. After a further two 5 min washes, immunoreactive bands

(10) TFF-1 Functions to Suppress Multiple Phenotypes Associated with Lung Cancer ProgressionOncoTargets and therapyJanuary 1, 2021Minegishi K, Dobashi Y, Tsubochi H, Hagiwara K, Ishibashi Y, Nomura S, Nakamura R, Ohmoto Y, Endo S.with alkaline-phosphatase-conjugated goat anti-rabbit (S3731, Promega, Madison, WI) or anti-mouse (S3721, Promega) secondary antibodies at a 1:1500 dilution for 1.5 hrs at room temperature (RT) followed by

(11) TIP60/P400/H4K12ac Plays a Role as a Heterochromatin Back-up Skeleton in Breast CancerCancer genomics & proteomicsNovember 1, 2020Idrissou M, Boisnier T, Sanchez A, Khoufaf FZH, Penault-Llorca F, Bignon YJ, Bernard-Gallon D.GAPDH Abs (1/5000, sc-47724, Santa Cruz Biotechnology). The secondary Abs: anti-mouse IgG (1/2000, S3721) and anti-rabbit IgG (1/2000, S3738) were purchased from Promega (Madison, WI, USA). RNA extraction,

(12) Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteinsPloS oneJanuary 1, 2020Oparija-Rogenmozere L, Rajendran A, Poncet N, Camargo SMR, Verrey F.conjugate (Cat# W4011, Lot# 0000340771, RRID:AB_430833) or anti-mouse IgG (H+L) AP conjugate (Cat# S3721, Lot# 0000312817, RRID:AB_430871) were used (both from Promega, Dübendorf, Switzerland). For immunofluorescence (IF

(13) Identification of the Virulence Factors of Candidatus Liberibacter asiaticus via Heterologous Expression in Nicotiana benthamiana using Tobacco Mosaic VirusInternational Journal of Molecular SciencesNovember 8, 2019Xiaobao Ying, Mengyuan Wan, Linshuang Hu, Jinghua Zhang, Hui Li, Dianqiu Lvtimes (each time for 5 min). The blocked membrane was incubated with AP-labelled secondary antibody S3721 (Promega) for one hour, followed by a thorough washing. The signal was detected with Western Blue® Stabilized Substrate for Alkaline Phosphatase

(14) Neuroprotective effect of a dietary supplement against glutamate-induced excitotoxicity in retinaInternational Journal of OphthalmologyAugust 18, 2019Takahiro Kurose, Eriko Sugano, Akihisa Sugai, Raki Shiraiwa, Mariyo Kato, Yoko Mitsuguchi, Yoshihiro Takai, Kitako Tabata, Yoichi Honma, Hiroshi TomitaJapan). Secondary antibodies against rabbit (Cat No. S3731) and mouse immunoglobulin G (IgG) (Cat No. S3721) were obtained from Promega (Tokyo, Japan). N-methyl-D-aspartate-induced Retinal Damage Retinal damage

(15) N-Methyl-N-Nitrosourea-Induced Photoreceptor Degeneration Is Inhibited by Nicotinamide via the Blockade of Upstream Events before the Phosphorylation of Signalling ProteinsBioMed Research InternationalApril 23, 2019Eriko Sugano, Kitako Tabata, Tsubasa Takezawa, Raki Shiraiwa, Hiroki Muraoka, Tomomi Metoki, Asaka Kudo, Yuki Iwama, Mitsuru Nakazawa, Hiroshi TomitaSanta Cruz Biotechnology. Secondary antibodies against rabbit (Cat No. S3731) and mouse immunoglobulin (Ig) G (IgG; Cat No. S3721) were obtained from Promega (Tokyo). 2.3. MNU and NAM Administration. Rats were administered a single

(16) d-amino acid oxidase promotes cellular senescence via the production of reactive oxygen speciesLife Science AllianceFebruary 1, 2019Taiki Nagano et al.antibody (W4011), HRP-conjugated anti-mouse antibody (W4021), and AP-conjugated anti-mouse antibody (S3721) were from Promega; anti-α-tubulin antibody (T9026) and anti-γ-tubulin antibody (T6557) were from Sigma-Aldrich;

(17) Structural and functional analysis of mRNA export regulation by the nuclear pore complexNature CommunicationsDecember 1, 2018Daniel H. Lin, Ana R. Correia, Sarah W. Cai, Ferdinand M. Huber, Claudia A. Jette, André Hoelzincubation at room temperature with an anti-mouse antibody coupled to alkaline phosphatase (Promega; S3721; 1:5000 dilution) visualized by color development using SIGMAFAST 5-bromo-4-chloro-3-indolyl phosphate

(18) De novo targeting to the cytoplasmic and luminal side of bacterial microcompartmentsNature CommunicationsDecember 1, 2018Matthew J. Lee, Judith Mantell, Ian R. Brown, Jordan M. Fletcher, Paul Verkade, Richard W. Pickersgill, Derek N. Woolfson, Stefanie Frank, Martin J. Warrensecondary antibody coupled to alkaline phosphatase (Anti-Mouse IgG (H + L), AP Conjugate (Promega; catalog #S3721; diluted 1:5000 in 50 mM Tris-HCl pH 7.5, 150 mM NaCl)). Bands were visualized by incubation in substrate

(19) SIRT1-dependent epigenetic regulation of H3 and H4 histone acetylation in human breast cancerOncotargetJuly 17, 2018Khaldoun Rifaï, Gaëlle Judes, Mouhamed Idrissou, Marine Daures, Yves-Jean Bignon, Frédérique Penault-Llorca, Dominique Bernard-Gallonthen washed and incubated with alkaline phosphatase-conjugated secondary Abs: anti-mouse IgG (1/2000, S3721) and anti-rabbit IgG (1/2000, S3738) (Promega, Madison, USA)). Immunolabeling was detected using Western Blue® Stabilized substrate for Alkaline Phosphatase

(20) Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cellsOncology lettersFebruary 1, 2018Nakagawa H, Sasagawa S, Itoh K.Promega Corporation, Madison, WI, USA) or AP-conjugated anti-mouse IgG (dilution, 1:7,500; cat. no. S3721; Promega Corporation) secondary antibodies for 30 min at room temperature. Final detection was performed

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Anti-Mouse IgG (H+L), AP Conjugate

Anti-Mouse IgG (H+L), AP Conjugate from Promega

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Anti-Mouse IgG (H+L), AP Conjugate from Promega

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