Fig 1: c-Abl is located at the synapse and its mRNA and protein levels increase after Aß oligomers (AßOs) treatment. (A) c-Abl, PSD95 and SAP102 protein levels increase as the neuronal culture ages (from 7 to 20 DIV). The graph shows quantification of protein levels. n = 3 (B) Immunofluorescence of hippocampal neurons showing c-Abl (in green) from soma to dendrites at 15 DIV. (C) Immunofluorescence showing c-Abl (in green) and PSD95 (in red) in hippocampal neurons at 19 DIV. The graph shows protein colocalization by Pearson’s correlation. Scale bar = 20 µm and 2 µm for magnifications. (D) Hippocampal cultured neurons were treated with 5 µM AßOs at indicated times. mRNA expression for Abl1 gene was assayed by RT-PCR using gapdh as loading control (water negative control). (E) Western-blot of c-Al total protein levels normalized to ßIII tubulin, as the graph shows (F). Neurons incubated with 5 µM fibrillary forms of the Aß-peptide (Aßf) were used as control. Unpaired t-test, *p < 0.5.