Fig 2: Sex and lineage differences in expression of a subset of voltage-gated ion channels. Immunofluorescent images showing expression of Slo2.2 (red) in GFP+ Dbx1-lineage or Foxp2-lineage MeA neurons (green; A). Insets show high-power magnification of GFP+ cells colocalized (white arrow) or not colocalized (open arrow) with Slo2.2. Graph of percentages of GFP+ Dbx1-lineage and Foxp2-lineage neurons in females and males expressing Slo2.2, with significant differences across sex observed in the Foxp2-lineage but not the Dbx1-lineage (B). Immunofluorescent images showing expression of KChip4.1 (C), Cav1.2 (E), Kv7.1 (G), and Kv1.1 (I; red) in GFP+ Dbx1-lineage or Foxp2-lineage MeA neurons (green). Insets show high-power magnification of GFP+ cells colocalized (white arrow) or not colocalized (open arrow) with each channel. Bar graph of percentages of GFP+ Dbx1-lineage and Foxp2-lineage neurons (male and female grouped) expressing KChip4.1 (D), Cav1.2 (F), Kv7.1 (H), or Kv1.1 (J), with significant differences observed across lineage but not sex (see also Extended Data Fig. 6-1) for Kv7.1 and Kir2.1; *p < 0.05, **p < 0.001, ***p < 0.0001. Lineage mRNA expression of additional voltage-gated ion channels is included in Extended Data Figure 6-2, with female and male comparisons in Extended Data Figure 6-3.