Fig 2: Absence of γδ T cells decreases angiogenesis. Scatter plots show the number of (a) endothelial cells (CD31+/CD45−) and (b) proliferating endothelial cells (CD31+/CD45−/BrdU+) per muscle fiber of ischemic gastrocnemius muscles of WT, ISO and γδ T cell depleted mice 7 days after femoral artery ligation (aFAL). Data are means ± SEM, n = 5 per group. * p < 0.05, ns ≥ 0.05 (WT vs. ISO vs. TCRγδ T cell depletion) by one-way ANOVA with the Tukey’s multiple comparisons test. (c) Representative images of ischemic gastrocnemius muscles of WT (top), isotype antibody-treated (middle) and TCRγδ T cell depleted mice (bottom) 7 days aFAL. Single channel pictures (small images) show endothelial cells (CD31, gray) and proliferating cells (BrdU, red). Merged images also show leukocytes (CD45, green) and nuclei (DAPI, blue). Scale bars: 30 µm.

Fig 3: TrkA agonism increases vascularity after extremity injury. Immunohistochemical stains of the vascular endothelial marker CD31, shown within representative sagittal sections of the proximal tenotomy site among Gambogic Amide (GA) and control-treated animals. Images shown at (a, b) 3 and (c, d) 9 weeks post-injury. (e) Quantification of CD31+ blood vessel volume among GA treated or control treated animals at 3 weeks post-injury. (f) Quantification of CD31+ blood vessel thickness among GA treated or control treated animals at 3 weeks post-injury. Yellow arrowheads indicate larger caliber blood vessels. Dashed white lines indicate the margins of the Achilles tendon. High magnification images are shown in yellow dotted frame. N = 5 animals per group. *P < .05, **P < .01. Scale bar: 100 µm.

Fig 4: Elevated cell adhesion molecule expression on SECs. a qPCR analysis of adhesion molecules, Selp, Sele, Icam1, and Vcam1 of sorted AECs and SECs. Data are represented as mean ± SEM. n = 4 for AECs and SECs. Data were analysed with two-tailed t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. b PDPN can be substituted for antibody against the adhesion molecule ICAM1 and E-selectin. Shown is EC isolation from a wild-type mouse injected i.v. with anti-PDPN and the enzymatically dissociated bone marrow cells were stained with other antibodies ex vivo and pre-gated on singlet, live, CD45−, Ter119−, CD31+ cells. c Anti-CD31 is dispensable for the analysis of bone marrow AECs and SECs. Flushed enzymatically dissociated bone marrow cells were stained with anti-CD45, anti-Ter119, anti-ICAM-1/anti-CD62E, anti-CD31, and anti-Sca-1 ex vivo. Analysis was pre-gated on singlet, live, CD45−, Ter119− cells. d P-selectin marks sinusoids. Anti-P-selectin and anti-CD31/CD144 were i.v. injected into Nestin-GFP mice and femur was imaged after removal. Scale bar, 50 um. e ICAM-1 marks sinusoids. Anti-ICAM-1 and anti-CD31/CD144 were i.v. injected into Nestin-GFP mice and femur was imaged after harvest. Scale bar, 100 um. f E-selectin marks sinusoids. Anti-E-selectin, anti-Sca-1, and anti-CD31/CD144 were i.v. injected into wild-type mice and femur was imaged after harvest. Scale bar, 50 um

Fig 5: Expression of Meflin in cells positive for conventional pericyte or PMC markers. (a) Flow cytometry of cells isolated from the kidneys of adult Meflin-CreERT2; LSL-tdTomato mice administered TAM (n = 3); 0.0086 ± 0.0012% of CD45−/CD31− living cells were positive for tdTomato (A). Histogram of tdTomato+ (red) and tdTomato− (blue) subsets stained with typical pericyte markers, including CD146 (Mcam), CD140a (Pdgfrα), CD140b (Pdgfrβ), and Sca1 (B). (b) UMAP plots showing distinct stromal cell populations (sC1–6) identified by single-cell RNA-seq of all stromal cells isolated from the mouse kidney (GEO accession number GSE129798) (A). r indicates the clustering resolution. Meflin (Islr) mRNA (indicated with an arrow) was expressed in the stromal cell subsets sC1, sC2, and sC4, overlapping with other stromal cell markers to varying degrees (B). Meflin was expressed in 33.8% of all stromal cells (C). Feature plots showing the expression of Meflin and other pericyte and PMC markers in the stromal cell subsets (D). Data were analyzed and visualized using R software47.