Fig 2: Ldh expression pattern in Imaginal discs.Representative confocal images of imaginal discs (top to bottom: wing disc, leg disc and eye disc) dissected from third instar (D5 AEL) Ldh-GFP genomic (A-F) and Ldh-GFPenhancer (G-L) third instar larvae. DAPI is shown in white and Ldh expression is shown in green. Note that the aLdh antibody failed to produce any staining in the imaginal discs and thus was excluded from the figure. The scale bar in all images represents 40 µM.

Fig 3: Characterization of Ldh expression in the intestine and Malpighian tubules.The Ldh expression pattern in the intestine and Malphigian tubules of L3 larvae was examined using Ldh-GFPGenomic, Ldh-GFPenhancer, and an aLdh antibody. (A-L) Representative confocal images of Ldh expression and DAPI staining in (A-F) the midgut and (G-L) the Malphigian tubules. Note that Ldh is expressed at significantly higher levels in (A,C,E) a subset of small cells of the AMP clusters (denoted with arrowheads in (A-F)) and (G,I,K) the stellate cells of the Malpighian tubules. For Ldh-GFPGenomic and Ldh-GFPenhancer expression analysis, tissues were fixed and stained with an aGFP antibody as described in the methods. The scale bar in all images represents 40 µM.

Fig 4: Ldh expression in ganglion mother cells, neurons, and glia.Representative confocal images showing the absence of Ldh in neuroblasts of third instar Ldh-GFP genomic larvae. The first two columns from left to right show DAPI and Ldh. The last column shows the colocalization of Ldh with CNS cell specific markers. (A-D) Ldh is not expressed in neuroblasts, which are labelled with aMiranda (MIRA) antibody. In contrast, (E-H) Ldh is expressed in ganglion mother cells (GMCs), which are labelled with aProspero (PROS) antibody. Ldh-GFP is also present in both (I-K) neurons stained with aElav and (M-P) and glia stained with aRepo. For Ldh-GFPGenomic and Ldh-GFPenhancer expression analysis, the CNS was fixed and stained with an aGFP antibody as described in the methods. The scale bar in all images represents 40 µM. Scale bar in (A) applies to B-D). Scale bar in (E) applies to (F-H). Scale bar in (I) applies to (J-L). Scale bar in (M) applies to (N-P).

Fig 5: Ldh expression pattern in the fat body and salivary gland.The Ldh expression patterns in the fat body and salivary glands of L3 larvae were examined using Ldh-GFPGenomic and Ldh-GFPenhancer. (A-L) Representative confocal images of Ldh expression and DAPI staining in (A-F) the fat body and (G-L) the salivary glands. For Ldh-GFPGenomic and Ldh-GFPenhancer expression analysis, tissues were fixed and stained with an aGFP antibody as described in the methods. The scale bar in all images represents 40 µM.