Fig 2: Expressions of SIRT1 (A), FOXO1 (B), and PGC1α (C) mRNA in rats subjected to MCAO/R. Data were expressed as means ± SD; n = 3. (D) Representative images of Western blot for SIRT1, FOXO1, PGC1α, and GAPDH. The quantitative analysis of the protein expressions of SIRT1 (E), FOXO1 (F), and PGC1α (G). Data were expressed as means ± SD; n = 3. ## p < 0.01 vs. the sham group; ** p < 0.01 vs. the MCAO/R group.

Fig 3: EX527 reversed the expression of protein and mRNA by HSYA and AHSYB in primary hippocampal cells. The relative mRNA expressions of Bax (A), Bcl-2 (B), SIRT1 (C), FOXO1 (D), and PGC1α (E). Data were expressed as means ± SD, n = 5. (F) Representative images of Western blot for Bax, Bcl-2, SIRT1, FOXO1, PGC1α, and GAPDH. The quantitative analysis of the protein expressions of Bax (G), Bcl-2 (H), SIRT1 (I), FOXO1 (J), and PGC1α (K). Data were expressed as means ± SD, n = 3. ## p < 0.01 vs. the control group; **p < 0.01 vs. the OGD/R group; ΔΔ p<0.01 vs. HSYA80; $ p < 0.05 vs. AHSYB80; $$ p < 0.01 vs. AHSYB80.

Fig 4: Expressions of SIRT1, FOXO1, and PGC1α in IHC. (A) SIRT1-, FOXO1-, and PGC1α-immunostained tissues of MCAO/R–induced rats (scale bar = 50 μm, magnification = ×200). The IHC assessments of SIRT1 (B), FOXO1 (C), and PGC1α (D) in MCAO/R–induced rats. Data were expressed as means ± SD; n = 3. ## p < 0.01 vs. the sham group; ** p < 0.01 vs. the MCAO/R group.