Fig 2: Normal development of T cell-dependent contact dermatitis in Il17c-/- mice. (a) Wild-type (n = 18) and Il17c-/- (n = 17) mice were epicutaneously sensitized and challenged with FITC or vehicle alone. The ear skin thickness of the mice was measured at the indicated time points after challenge. (b) Wild-type (n = 15) and Il17c-/- (n = 17) mice were epicutaneously sensitized and challenged with DNFB or vehicle alone. The ear skin thickness of the mice was measured at the indicated time points after challenge. (c) Representative images of skin sections (from 24 hours in (a) and (b)) stained with hematoxylin and eosin. ×100. The data show the mean + SEM (a and b).

Fig 3: Normal development of T cell-independent pulmonary fibrosis in Il17c-/- mice. Male mice were treated intratracheally with bleomycin (BLM) or PBS. 14 days later, bronchoalveolar lavage (BAL) fluids and the lungs were collected. (a) Cell counts in BAL fluids. Wild-type mice (PBS, n = 5; BLM, n = 9) and Il17c-/- mice (PBS, n = 5; BLM, n = 7). The data show the mean + SEM. **p < 0.01 and ***p < 0.001: PBS vs BLM; and †p < 0.05: wild-type mice vs. Il17c-/- mice. (b,c) Representative images of lung sections. Hematoxylin and eosin staining (b) and Elastica van Gieson staining (c). Bars = 100 µm.

Fig 4: Normal development of T cell- and IL-17A-independent airway neutrophilia in Il17c-/- mice. Mice were treated intratracheally with LPS or saline. 24 hours later, bronchoalveolar lavage (BAL) fluids were collected. (a)Cell counts in BAL fluids. Wild-type mice (saline, n = 5; LPS, n = 10) and Il17c-/- mice (LPS, n = 5). (b) The levels of cytokines in the BAL fluids in (a). The data show the mean + SEM. ***p < 0.001 vs. wild-type mice.

Fig 5: Normal development of T cell-dependent hepatitis in Il17c-/- mice. Female mice were injected intravenously with concanavalin A (Con A). 24 hours later, sera and livers were collected. (a) Serum levels of GOT and GPT. Wild-type mice (Saline, n = 4; Con A, n = 5) and Il17c-/- mice (Saline, n = 3; Con A, n = 5). The data show the mean + SEM. NS = not significant. (b) Representative images of liver sections stained with hematoxylin and eosin. Bars = 20 µm.