Fig 2: (A) Tag-free rhMFG-E8 has high biological activity. PS binding (left panel) and SVEC4-10 cell adhesion assay (right panel) of commercial MFG-E8 (R&D MFG-E8) and tag-free rhMFG-E8 (rhMFG-E8). (B-C) Tag-free rhMFG-E8 is highly stable. PS binding and cell adhesion assays performed at (B) twelve months, (C) twenty-four months after storage of the tag-free rhMFG-E8 at -20°C. D. Tag-free rhMFG-E8 has suitable pharmacokinetics in healthy mice. Rats were iv injected with tag-free MFG-E8 and blood samples collected at different timepoints. The levels of the tag-free rhMFG-E8 were measured using human MFG-E8 Quantikine ELISA kit. The data were plotted as serum rhMFG-E8 concentration against time points to determine the t1/2.