Fig 2: Impact of gene silencing of talin (Tln-1) and ß1 integrin (Itgb1) on the motility and transmigration of T. gondii-challenged DCs. a Tln1 mRNA expression (2-?Ct) in DCs transduced with shTln1 or control shLuc lentivirus relative to mock-treated cells (mock set to 1.0). b Itgb1 mRNA expression (2-?Ct) in DCs transduced with shITGB1 or control shLuc lentivirus relative to mock-treated cells. c Representative motility plots of mock-treated DCs and DCs transduced with lentiviral vectors targeting Itgb1 mRNA (shITGB1), Tln1 mRNA (shTln1) or a non-expressed target (shLuc) challenged with T. gondii, on collagen. d Box-and-whisker and scattered dot plots represent median velocities (µm/min) of DCs on collagen, as in (c). Grey circles represent velocities from individual cells. e Representative motility plots of mock-treated DCs and DCs transduced with lentiviral vectors targeting Itgb1 mRNA (shITGB1), Tln1 mRNA (shTln1) or a non-expressed target (shLuc) challenged with T. gondii, on bEnd.3 monolayers. f Box-and-whisker and scattered dot plots represent median velocities (µm/min) of DCs on bEnd.3, as in e. Grey circles represent velocities from individual cells. g Bar graphs show percentage (%) of transduced DCs adhered to bEnd.3 monolayers related to DCs in the same frame in static condition, following fluidic shear stress (0.2 dyn/cm2). h, i Box-and-whisker and scattered dot plots represent median pathlengths (µm) and velocities (µm/s), respectively, of tracked cells on bEnd.3 monolayers. Grey circles represent pathlengths or velocities, respectively, from individual cells. j, k Permeability to FITC-dextran (3 kDa) and TEER (%), respectively, of bEnd.3 cell monolayers following DC transmigration. l TEM frequency shown as percentage (%) of DCs added in the upper well. Bar graphs show mean ± s.e.m. and box plots show median from 3–4 independent experiments (n = 3–4). In box-and-whisker plots, boxes mark 25th to 75th percentile and whiskers mark 10th and 90th percentiles of the datasets. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, non-significant by Students’ t test (a, b), one-way ANOVA, Dunnett’s post hoc test (d, f), Kruskal–Wallis, Dunn’s post hoc test (h, i), repeated measures one-way ANOVA, Sidak’s post hoc test (j, k, l)