Fig 1: Identification and validation of the promoter region DNA methylation marker. A, D, G, J Percentages of TBX3 (A), AKT3 (D), SOX2 (G), SGK1 (J) DNA methylation level of adrenocortical adenoma tissue between non-pregnant and pregnant groups. B, E, H, K Immunohistochemical staining of adrenocortical adenoma tissue with anti-TBX3 (B), AKT3 (E), SOX2 (H), SGK1 (K) antibodies between non-pregnant and pregnant groups. C, F, I, L Statistical analysis of figure (B, E, H, K). Statistical significances are represented by asterisks *P < 0.05; **P < 0.01
Fig 2: YTHDF1 facilitated the proliferation of HCC cells by activating PI3K/AKT/mTOR signaling pathway. A The results of GSEA indicated that PI3K/AKT/mTOR signaling pathway was enriched in HCC with YTHDF1-overexpression. B The correlation analysis between YTHDF1 and key molecules of PI3K/AKT/mTOR signaling pathway in HCC was performed by using GEPIA. C Western blot was performed to detect the protein levels of AKT, p-AKT, mTOR, p-mTOR in Huh7 and MHCC-97H cells with YTHDF1 knockdown. D Western blot was performed to detect the protein levels of AKT1, AKT2, and AKT3 in MHCC-97H cells with YTHDF1 silencing. E q-RT PCR was performed to measure the mRNA levels of AKT1, AKT2, and AKT3 in Huh7 and MHCC-97H cells with YTHDF1 silencing. F YTHDF1-deficient MHCC-97H cells treated with 4ug/ml SC79 for 1 h, cell proliferation was measured by Edu assay. Representative images and quantitative histograms were shown. *P < 0.05, **P < 0.01
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