Fig 2: SAP-EV abundantly express ITGAM and ITGB2. Extracellular vesicles were extracted from the serum of the three groups mice and analyzed by proteomics. (A) Principal component analysis (PCA) of three groups of EVs (SC-EV, MAP-EV, and SAP-EV) proteomics. (B) Volcano diagram of protein expression differences between SAP-EV and MAP-EV group. (C) GO enrichment analysis of protein expression differences between SAP-EV and MAP-EV group (*p < 0.05, **p < 0.01, ***p < 0.001). (D) Heat map and statistical analyses of integrin protein expression in three groups EVs (n = 3/group; *p < 0.05, ***p < 0.001, ****p < 0.0001 vs SC-EV, ###p< 0.001, ####p < 0.0001 vs SAP-EV). (E) qPCR was used to detect the mRNA levels of integrin-related proteins ILK, ITGA2B, ITGAM, and ITGB2 in the three groups of EVs (n = 6/group; ***p < 0.001, ****p < 0.0001 vs SC-EV, #p < 0.05, ##p < 0.01, ####p < 0.0001 vs SAP-EV).