Fig 1: IHC and survival analysis of MC markers.a Representative images of IHC staining of CEACAM6, REG4, FCGBP, SPINK4 and MUC2 expression in paired MC or AC cancer tissues and corresponding normal tissues. Scale bar, 100 μm. b Differential analysis of CEACAM6, REG4, FCGBP, SPINK4 and MUC2 levels in paired MC cancer tissues and normal tissues (n = 9 biologically independent patients) and AC cancer tissues and normal tissues (n = 9 biologically independent patients). Statistical analyses were performed by a nonparametric test followed by the Mann‒Whitney test. All the bars represent the mean ± S.D. c Kaplan‒Meier survival curves were generated for the top 20 DEGs by comparing groups of high (red line) and low (blue line) gene expression. The dotted line represents the 95% confidence interval. p < 0.05 according to the log-rank test.