Fig 1: The effects of butyrate treatment on the expression of MTCO1, MTCO2, and COX4 in NSC34-G93A cells. NSC34 cells were transfected with mt-SOD1G93A-GFP. The incubation of 1 mM butyrate was started 24 h after the transfection. RNA was extracted after 4, 8, and 48 h post the butyrate treatment. (A–C, left panels): mRNA levels of MTCO1, MTCO2, and COX4 were determined by RT-qPCR and normalized to the levels in cells without the butyrate treatment. n = 3. (A–C, right panels): Relative protein levels of MTCO1, MTCO2, and COX4 with/without butyrate treatment from 1 to 4 days were analyzed using ImageJ with actin as a reference. Representative immunoblotting images are shown. n = 4. For the box and dot plot, the box bottom, median line, and box top represent the 25th (Q1), 50th (Q2), and 75th (Q3) percentiles, respectively. Whisker ends represent Q1 - 1.5*IQR and Q3 + 1.5*IQR, respectively. IQR is the interquartile range (Q3 - Q1). * p < 0.05; ** p < 0.01; NS: not significant.