Fig 2: Differential expression validation of 7 CPGs and 2 potential CPGs between KIRC and normal samples. GEPIA database was used to compare the expression differences of FDX1 (A), ACADM (D) and ACAT1 (G) between KIRC and normal kidney tissues. The HPA database showed the expression of FDX1 (B), ACADM (E) and ACAT1 (H) at the tissue protein level by immunohistochemistry. Western Blot was used to compare the protein levels of FDX1 and ACAT1 in KIRC cells (Caki-1 and 786-0) and normal renal tubular epithelial cells (HK-2), and the results were semi-quantified by integrated density (J). qRT-PCR was used to compare mRNA levels of FDX1 (C), ACADM (F), ACAT1 (I), PDHA1 (K), PDHB (L), DLAT (M), DLD (N), LIAS (O) and LIPT1 (P) in KIRC cells (Caki-1, 786-0) and normal renal tubular epithelial cells (HK-2). CPG, cuproptosis-promoting gene; N, normal; T,tumor; ns, no significant difference, *p < 0.05, **p < 0.01, and ****p < 0.0001. Western Blot data are means ± SD, with n = 3; qRT-PCR data are means ± SD, with n = 4.