Fig 2: Silence Plin5 or GW6471 could reverse the protective effect of dapagliflozin-mediated PPARa signaling axis in AngII-induced cardiomyocyte hypertrophy. The content of ANP (A), BNP (B), ß-MHC (C) and cTNI (D) in cell culture supernatant was detected by ELISA kits. qRT-PCR analysis was performed to detect the mRNA expression of Nppa (E), Nppb (F), Serca2 (G) and myh7 (H) in different groups. (I–L) Western blotting assay was used to detect the protein expressions of Plin5, PPARa, HMGCS2 and PDK4 in cardiomyocyte of different groups. The data were expressed as mean ± SD, n = 3, *p < 0.05, vs. AngII + DAPA + DMSO group; #p < 0.05, vs. AngII + DAPA + si-NC group.

Fig 3: Dapagliflozin mediated the Plin5/PPARa signaling axis to mitigate AngII-induced cardiomyocyte hypertrophy. qRT-PCR analysis was used to detect the mRNA expressions of STAT1 (A), Plin5 (B), PPARa (C), HMGCS2 (D) and PDK4 (E) in cardiomyocytes of different groups. (F–J) Western blotting assay was performed to detect the protein expressions of STAT1, Plin5, PPARa, HMGCS2 and PDK4. The data were expressed as mean ± SD, n = 3, *p < 0.05, vs. control group; #p < 0.05, vs. AngII group.