Anti-PADI2 / PAD2 antibody ab16478 from Abcam

Product Specs
ItemAnti-PADI2 / PAD2 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab16478
Antibody TypePrimary
HostRabbit
Quantity100 UG
IsotypeIgG
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Gene NamePADI2
ReactivityHuman
TargetPADI2 / PAD2

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Citrullination is linked to reduced Ca 2+ sensitivity in hearts of a murine model of rheumatoid arthritisActa Physiol (Oxf)November 1, 2022Gianluigi Pironti, Stefano Gastaldello, Dilson E. Rassier, Johanna T. Lanner, Mattias Carlström, Lars H. Lund, Håkan Westerblad, Takashi Yamada, Daniel C. Anderssontransferred onto PVDF membranes. Membranes were incubated with primary antibodies: anti‐PAD2 (1:1000, Abcam, ab16478), anti‐RyR2 (1:5000, Abcam, ab2728), anti‐phosphorylated‐S2808‐RyR (1:5000, donated by Andrew R Marks,

(2) Subjects at-risk for future development of rheumatoid arthritis demonstrate a PAD4- and TLR-dependent enhanced histone H3 citrullination and proinflammatory cytokine production in CD14hi monocytesJournal of AutoimmunityFebruary 1, 2021Yuko Okamato et al.(Abcam, ab1791), cit-H3 (citrulline R2+R8+R17, Abcam, ab5103), PAD4 (Abcam, ab128086), PAD2 (Abcam, ab16478), and β-actin (Cell Signaling, 3700). Secondary antibodies included IRDye 680RD Donkey anti-mouse IgG

(3) Identification of Conserved Proteomic Networks in Neurodegenerative DementiaCell ReportsJune 23, 2020Vivek Swarup, Timothy S. Chang, Duc M. Duong, Eric B. Dammer, Jingting Dai, James J. Lah, Erik C.B. Johnson, Nicholas T. Seyfried, Allan I. Levey, Daniel H. GeschwindAnti-PLCD1 antibody (Abcam #ab134936), anti-HEPACAM (Abcam #ab130769), anti-PADI2 antibody (Abcam #ab16478), anti-GAPDH antibody (Abcam #ab8245), and anti-Connexin-43 (Sigma #C6219) overnight at 4°C. Membranes

(4) Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus InfectionFrontiers in immunologyJanuary 1, 2020Casanova V, Sousa FH, Shakamuri P, Svoboda P, Buch C, D'Acremont M, Christophorou MA, Pohl J, Stevens C, Barlow PG.Sigma-Aldrich (Dorset, UK). Antibodies Antibodies against Citrullinated Histone H3 (ab5103), PAD2 (ab16478), PAD4 (ab128086), PAD3 (ab183209), Tubulin and β-actin (ACTN05) were purchased from Abcam (Cambridge,

(5) Spontaneous Secretion of the Citrullination Enzyme PAD2 and Cell Surface Exposure of PAD4 by NeutrophilsFrontiers in ImmunologySeptember 25, 2017Yebin Zhou et al.(73, 74). Antibodies and Reagents Antibodies for detection of PAD4 (Ab128086 and Ab50247), PAD2 (Ab16478), citrullinated histone H3 (R2 citrullination, Ab176843), and total histone H3 (Ab24834) were from Abcam

(6) Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myelomaLeukemiaFebruary 1, 2017G McNee et al.antibodies against actin (AC-40; Sigma), R26cit (ab19847; Abcam), R2,8,17cit (ab5103; Abcam), PADI2 (ab16478; Abcam) or PADI4 (ab128086; Abcam), followed by the appropriate horseradish peroxidase-linked secondary

(7) Rheumatoid arthritis antigens homocitrulline and citrulline are generated by local myeloperoxidase and peptidyl arginine deiminases 2, 3 and 4 in rheumatoid nodule and synovial tissueArthritis research & therapyOctober 20, 2016Turunen S, Huhtakangas J, Nousiainen T, Valkealahti M, Melkko J, Risteli J, Lehenkari P.antigen retrieval in citrate buffer pH 6. PAD2 enzyme was stained with anti-human PAD2 antibody 1:250 (ab16478, Abcam) and PAD3 with 1:50 (ab183209, Abcam) after heat-mediated antigen retrieval in Tris-EDTA, pH

(8) Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte GlycoproteinJournal of immunology (Baltimore, Md. : 1950)August 15, 2016Jagessar SA, Holtman IR, Hofman S, Morandi E, Heijmans N, Laman JD, Gran B, Faber BW, van Kasteren SI, Eggen BJ, et al.incubated with the following primary Abs in PBS/1% BSA for 1 h at room temperature: anti-rabbit PAD2 (1:28; ab16478; Abcam, Cambridge, U.K.), anti-rabbit PAD4 (1:10; PA5-22317; Invitrogen), and anti-mouse LC3 (1:250;

(9) Mechanical Stretching Elevates Peptidyl Arginine Deiminase 2 Expression in AstrocytesCurrent Eye ResearchApril 11, 2014Mabel E. Algeciras, Sanjoy K. Bhattacharya, Sanjoy K. BhattacharyaA and B incubation step was omitted and a rabbit polyclonal antibody against PAD2 (Abcam Inc., CA, ab16478) was used for detection using an identical procedure. Purified BSA and recombinant purified PAD2 (5

(10) Retinal deimination and PAD2 levels in retinas from donors with age-related macular degeneration (AMD)Experimental Eye ResearchJune 1, 2013Vera L. Bonilha, Karen G. Shadrach, Mary E. Rayborn, Yong Li, Gayle J. T. Pauer, Stephanie A. Hagstrom, Sanjoy K. Bhattacharya, Joe G. Hollyfield(#17–347, from the Anti-Citrulline (modified) Detection Kit, EMD Millipore, Lake Placid, NY) and PAD2 (ab16478, Abcam, Cambridge, MA) in Blotto A buffer (20 mM Tris/HCl, 0.9% NaCl, 0.05% Tween 20 (TBST), 5% skimmed milk)

(11) PADI2 is significantly associated with rheumatoid arthritisPloS oneJanuary 1, 2013Xiaotian Chang, Yifang Xia, Jihong Pan, Qingsong Meng, Yan Zhao, Xinfeng Yan

(12) Peptidylarginine deiminases present in the airways during tobacco smoking and inflammation can citrullinate the host defense peptide LL-37, resulting in altered activitiesAmerican journal of respiratory cell and molecular biologyFebruary 1, 2012Ola Kilsgård, Pia Andersson, Martin Malmsten, Sara L Nordin, Helena M Linge, Mette Eliasson, Eva Sörenson, Jonas S Erjefält, Johan Bylund, Anders I Olin, Ole E Sørensen, Arne Egesten

(13) Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic nerveMol VisAugust 17, 2010Thamara A. Cafaro, Stefanía Santo, Lucena A. Robles, Nicolás Crim, Julio A. Urrets-Zavalia, Horacio M. SerraPAD2, the reagent A and B incubation step was omitted and a rabbit polyclonal antibody against PAD2 (ab16478; Abcam Inc.) was used for detection using an identical procedure. Purified CRALBP alone and recombinant

(14) Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritisArthritis and rheumatismAugust 1, 2008Andrew Kinloch, Karin Lundberg, Robin Wait, Natalia Wegner, Ngee Han Lim, Albert J W Zendman, Tore Saxne, Vivianne Malmström, Patrick J Venables

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Anti-PADI2 / PAD2 antibody from Abcam

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Anti-PADI2 / PAD2 antibody from Abcam

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