Fig 1: β2-and β3-AR antagonists reduce neuroinflammation in tibial nerve of K7M2 osteosarcoma-bearing mice (A) Representative flow cytometry analysis and relative quantification of F4/80+/CD64+ macrophages (B) typical immunofluorescence images and pooled data of F4/80+ cells and (C) H2O2 content in the tibial nerve after para-tibial K7M2 osteosarcoma cell inoculation or sham in BALB/c mice treated (daily, starting from day 10) with atenolol (10 mg/kg, i.p.) propranolol (10 mg/kg, i.p.), SR59230A (10 mg/kg, i.p.) or vehicle (Veh) (D) H2O2 level in RAW 264.7 stimulated with PMA (16 μM) in presence of SR59230A, propranolol, atenolol (all, 100 nM) or Veh (n = 3 independent experiments) (E) Mechanical allodynia at day 14 after para-tibial K7M2 osteosarcoma cell inoculation or sham in BALB/c mice treated with PBN (100 mg/kg, i.p.) or Veh. BL, baseline. PWT, paw withdrawal threshold. N = 6 mice. *p < 0.05, ***p < 0.001 vs Sham, Veh-Veh; § p < 0.05, §§§ p < 0.001 vs K7M2 + Veh, Veh-PMA, K7M2 + Veh PBN. Data are presented as mean ± SEM. One-way (A–C) or two-way (D–E) ANOVA and Bonferroni post hoc test.