Fig 1: PRMT4 positively regulates the AKT/mTOR signaling pathway. (A) The expression levels of AKT, p-AKT, mTOR, p-mTOR, RPS6, p-RPS6, 4E-BP1 and p-4E-BP1 were detected by western blot in stable cell lines. (B) The inhibition of the AKT/mTOR pathway abolished the cell proliferation mediated by PRMT4 overexpression, as indicated by CCK-8 assays. (C) The inhibition of the AKT/mTOR pathway abolished the cell migration and invasion mediated by PRMT4 overexpression, as indicated by Transwell assays (×400 magnification). (D) Calculation of cells that migrated and invaded through the filter in YY-8103 cells. Measurement data were expressed as mean ± SD, and the experiments were repeated at least 3 times. *P < 0.05, **P < 0.01vs PRMT4 group.