Fig 1: Sev impaired the tight junction of blood-testis barrier. (A) and (B) Sev inhibited ZO1 expression in testis by Western blot detection. (C) and (D) Effects of Sev on ZO1 expression in epididymis. (E) and (F) The co-expression of DDX4 and ZO-1 was detected by immunofluorescence in testis and epididymis. Data are expressed as mean ± SD. *p < 0.05 compare to that of control group.