Fig 2: Inhibition of NLRC5 increases the sensitivity of GC cells to 5-FU. (A) The expression of NLRC5 was detected by western blotting. ***P<0.001 vs. AGS. (B) Cell apoptosis was detected by flow cytometry. (C) Western blotting detected the expression of apoptosis-related proteins. ***P<0.001 vs. control, #P<0.05, ##P<0.01, ###P<0.001 vs. 5-FU + siRNA-NC. NLRC5, NOD like receptor family, CARD domain containing 5; GC, gastric cancer; 5-FU, 5-fluorouracil.

Fig 3: Inhibition of NLRC5 reduces invasion and migration of GC cells. (A) Wound healing detected the ability of cell migration (magnification, x100). (B) Transwell detected the ability of cell invasion (magnification, x100). (C) Western blotting detected the expression of MMP2 and MMP9. **P<0.01, ***P<0.001 vs. siRNA-NC. NLRC5, NOD like receptor family, CARD domain containing 5; GC, gastric cancer; si, short interfering; NC, negative control.

Fig 4: YY1 binds to the promoter of NLRC5. (A) JASPAR website was used to predict the binding between YY1 and NLRC5. (B) RT-qPCR was used to detect the expression of YY1. (C) Western blotting was used to detect the expression of YY1. (D) RT-qPCR was used to detect the expression of NLRC5 following cell transfection. (E) Western blotting was used to detect the expression of NLRC5 following cell transfection. *P<0.05, ***P<0.001 vs. pc-DNA-NC. ###P<0.001 vs. siRNA-NC. (F) Promoter activity of NLRC5 was detected by luciferase. ***P<0.001 vs. Ov-NC. (G) Chromatin immunoprecipitation detected the binding ability of YY1 and NLRC5 promoter. ***P<0.001 vs. IgG. YY1, Yin Yang 1; NLRC5, NOD like receptor family, CARD domain containing 5; RT-qPCR, reverse transcription-quantitative PCR; si, short interfering; NC, negative control; Ov, overexpression; FL, full length.

Fig 5: Inhibition of NLRC5 blocks the promotion of YY1 on proliferation, invasion and migration. (A) CCK-8 detected the cell viability. (B) Colony formation assay was used to detected the cell proliferation. (C) Wound Healing detected the ability of cell migration (magnification, x100). (D) Transwell detected the ability of cell invasion (magnification, x100). (E) Western blotting detected the expression of MMP2 and MMP9. *P<0.05, **P<0.01, ***P<0.001 vs. Ov-NC. #P<0.05, ##P<0.01, ###P<0.001 vs. Ov-YY1+siRNA-NC. NLRC5, NOD like receptor family, CARD domain containing 5; YY1, Yin Yang 1; Ov, overexpression; NC, negative control; si, short interfering.