Carbonic Anhydrase IV/CA4 Antibody AF2414 from R&D Systems, a Bio-Techne Brand

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Scientific reports
February 06 2020

Fig 1: Summary of the patterns of co-expression between protocadherin-20 (Pcdh20) and taste cell markers in taste cells of the circumvallate papillae. The areas of the circles indicate the numbers of taste bud cells expressing Pcdh20, taste receptor type 1 member 3 (T1R3, a sweet or umami taste cell marker), gustducin (Gust, a G protein mediating mainly bitter taste transduction in mouse circumvallate papillae19) and carbonic anhydrase-4 (Car4, a sour taste cell marker) in the circumvallate papillae of mice. The overlapped area indicates the co-expression. This diagram is based on Table 1.
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eLife
May 19 2021

Fig 2: KRT14+ progenitor contribution of new cells to taste buds begins at birth.(A) Quantification of KRT8+ cells per FFP section reveals taste bud cell number does not increase until P9. Blue bars: mean ± SD (n = 3 animals per stage, 8–18 taste buds per animal, open and shaded shapes) Student’s t-test ***p<0.001. (B–E) In pups that received EdU at P0, P7, or P9, analysis at 48 hr revealed comparable proportions of taste buds housed newly generated EdU+/KRT8+ cells (yellow arrows in C–E) regardless of postnatal day of labeling (EdU green, KRT8 magenta). (F–I) Lineage tracing with Krt14CreERT2; R26RYFP (KRT14-YFP) initiated at P0, P7, or P14 assessed at 48 hr showed extensive YFP expression (green) in FFP non-taste epithelium as well as YFP+/KRT8+ cells in taste buds (magenta, yellow arrows in G–I). (C–E, G–I) Dashed lines delimit the basement membrane; solid lines delimit the epithelial surface. (B, F) Blue bars: mean ± SD Student’s t-test **p<0.005 (B: n = 6 animals per stage, 14–28 taste buds per animal; F: n = 3 animals per stage, 10–24 taste buds per animal). (J–M) SHH+ taste precursor cells are not lineage restricted. ShhCreERT2; R26RtdTomato (SHH-Tomato, green) mice treated with TAM at E12.5 reveals similar proportions of type III (NCAM+, CAR4+ magenta in K, L) and type II (PLCß2+ magenta in M) taste cells are tomato+ (green) (N = 3 mice, counts from 24 NCAM+, 30 CAR4+ and 30 PLCß2+ total TBs). (N–Q) Postnatally activated KRT14+ progenitors are not lineage restricted. Krt14CreERT2;R26RYFP (K14-YFP, green) mice treated with TAM at P1 labels both type III (NCAM+, CAR4+ magenta in O, P) and type II (PLCß2 magenta in Q). Double labeled cells indicated with yellow arrows in all image panels. Dashed lines delimit the basement membrane; solid lines delimit the epithelial surface.
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Science advances
November 18 2022

Fig 3: Cocaine withdrawal increases CA4 expression in the NAc.(A) Representative Western blot of CA4 and ß-actin in protein lysates of NAc tissue punches from mice of indicated genotypes and treatment groups. (B) Cocaine withdrawal increased CA4 protein in NAc relative to saline-treated controls [t(12) = 3.575, **P = 0.0038, and n = 7 per group], and CA4 was absent in Car4-/- NAc.
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Science advances
November 18 2022

Fig 4: Cocaine withdrawal and CA4 disruption–induced changes in dendritic spines.(A) Representative images of dendritic spines. (B) Cocaine withdrawal increased total spine density in Car4+/+ mice and also in Car4-/- mice but not to same levels [interaction, F(1, 74) = 3.66 and P = 0.059; treatment, F(1, 74) = 35.21 and P < 0.0001; genotype, F(1, 74) = 88.71 and P < 0.0001, n = 18 to 20; planned contrasts: Coc versus Sal Car4+/+, ****P < 0.0001 and n = 20 neurons from five mice and 18 neurons from five mice, respectively; Coc versus Sal Car4-/-, **P = 0.0052 and n = 20 neurons from five mice and 20 neurons from five mice, respectively; Car4+/+ versus Car4-/- Sal, ****P < 0.0001; Car4+/+ versus Car4-/- Coc, ****P < 0.0001]. Sample sizes are the same from (D) to (F). (C) Examples of stubby, thin, and mushroom spines. (D) Thin spines [interaction, F(1, 74) = 2.377 and P = 0.127; treatment, F(1, 74) = 31.31 and P < 0.0001; genotype, F(1, 74) = 72.16 and P < 0.0001; planned contrasts: Coc versus Sal Car4+/+, ****P < 0.0001; Coc versus Sal Car4-/-, **P = 0.0048; Car4+/+ versus Car4-/- Sal, ****P < 0.0001; Car4+/+ versus Car4-/- Coc, ****P < 0.0001]. (E) Stubby spines. [interaction, F(1, 74) = 0.85 and P = 0.35; treatment, F(1, 74) = 27.97 and P < 0.0001; genotype, F(1, 74) = 47.53 and P < 0.0001; planned contrasts: Coc versus Sal Car4+/+, **P = 0.0032; Coc versus Sal Car4-/-, ****P < 0.0001; Car4+/+ versus Car4-/- Sal, ****P < 0.0001; Car4+/+ versus Car4-/- Coc, ****P < 0.0001]. (F) Mushroom spines [interaction, F(1, 74) = 7.95 and P = 0.006; treatment, F(1, 74) = 4.189 and P = 0.04; genotype, F(1, 74) = 1.358 and P = 0.24; planned contrasts: Coc versus Sal Car4+/+, P = 0.59; Coc versus Sal Car4-/-, ***P = 0.0008; Car4+/+ versus Car4-/- Sal, **P = 0.006; Car4+/+ versus Car4-/- Coc, P = 0.23].
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Science advances
November 18 2022

Fig 5: Disrupting CA4 prevented cocaine withdrawal–induced increases in mEPSC amplitude, decay time, and frequency in NAc MSNs.(A) Representative traces of mEPSCs from Car4+/+ and Car4-/- mice after cocaine withdrawal (Coc-WD) and saline withdrawal (Sal-WD). (B) Cocaine withdrawal (Coc) increased mEPSC amplitude in Car4+/+ but not in Car4-/- mice [interaction, F(1, 106) = 13.73 and P = 0.0003; planned contrasts: Coc versus Sal Car4+/+, **P = 0.0011 and n = 27 neurons from seven mice and 17 neurons from five mice, respectively; Coc versus Sal Car4-/-, P = 0.084 and n = 29 neurons from seven mice and 37 neurons from eight mice, respectively]. (C) Cocaine withdrawal reduced mEPSC decay time in Car4+/+ but not in Car4-/- mice [interaction, F(1, 108) = 13.15 and P = 0.0004; planned contrasts: Coc versus Sal Car4+/+, ****P < 0.0001; Coc versus Sal Car4-/-, P = 0.83, sample sizes as in (B)]. (D) Cocaine withdrawal increased mEPSC frequency in Car4+/+ mice but not in Car4-/- mice [interaction, F(1, 105) = 6.237 and P = 0.0004; planned contrasts: Coc versus Sal Car4+/+, **P = 0.0042; Coc versus Sal Car4-/-, P = 0.83, sample sizes as in (B)].

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Carbonic Anhydrase IV/CA4 Antibody

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R&D Systems, a Bio-Techne Brand's Carbonic Anhydrase IV/CA4 Antibody is a Goat Polyclonal antibody. This antibody has been shown to work in applications such as: Simple Western, Immunohistochemistry, Immunoprecipitation, and Western Blot. The Carbonic Anhydrase IV/CA4 Antibody was generated using Car4, and carbonic anhydrase 4 as the antigen and it reacts with Mouse.

Product Specs
ItemCarbonic Anhydrase IV/CA4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF2414
NCBI Gene AliasesCa4
ApplicationsWestern Blot, Simple Western, Immunohistochemistry, Immunoprecipitation
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetCarbonic Anhydrase IV/CA4
HostGoat
Conjugate/TagUnconjugated
ReactivityMouse
NCBI Full Gene Namecarbonic anhydrase 4
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4, Glu18-Ser277, Accession # Q64444
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CA4 is observed

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(1) Adrenomedullin Enhances Mouse Gustatory Nerve Responses to Sugars via T1R-Independent Sweet Taste PathwayNutrientsJune 28, 2023Iwata S, Yoshida R, Takai S, Sanematsu K, Shigemura N, Ninomiya Y.GFP-1020, Aves Labs, Inc., Davis, CA, USA), anti-carbonic anhydrase 4 (CA4) (1:100; goat anti-CA4, cat. no. AF2414, R&D Systems, Minneapolis, MN, USA), anti-CRLR (1:300; rabbit anti-T1R3, cat. no. bs-1860R, Bioss Antibodies,

(2) Endothelial progenitor cells derived from embryonic stem cells prevent alveolar simplification in a murine model of bronchopulmonary dysplasiaFrontiers in Cell and Developmental BiologyJune 9, 2023Olena A. Kolesnichenko, Hannah M. Flood, Yufang Zhang, Vladimir Ustiyan, Hayde K. Cuervo Jimenez, Tanya V. Kalin, Vladimir V. KalinichenkoEMD Millipore; AB5320A4), GPIHBPI (1:300; Thermofisher Scientific; PA5-16976), CAR4 (1:250; R&D Systems; AF2414). Changes in alveolar size were determined as previously described (Xia et al., 2015) using the morphometric

(3) Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to NutrientsCellular and molecular gastroenterology and hepatologyJanuary 1, 2023McCauley HA, Riedman AM, Enriquez JR, Zhang X, Watanabe-Chailland M, Sanchez JG, Kechele DO, Paul EF, Riley K, Burger C, et al.,R&D Systems,AF3437,Goat,1:200 Chromogranin A,ImmunoStar,20085,Rabbit,1:200 Carbonic anhydrase 4,R&D Systems,AF2414,Goat,1:200 Ki67,Cell Marque,275R-14,Rabbit,1:200 Cleaved caspase-3,Cell Signaling Technology,9661S,Rabbit,1:200 Defensin

(4) Bisphosphonate affects the behavioral responses to HCl by disrupting farnesyl diphosphate synthase in mouse taste bud and tongue epithelial cellsScientific reportsDecember 8, 2022Oike A, Iwata S, Hirayama A, Ono Y, Nagasato Y, Kawabata Y, Takai S, Sanematsu K, Wada N, Shigemura N.

(5) Carbonic anhydrase 4 disruption decreases synaptic and behavioral adaptations induced by cocaine withdrawalScience advancesNovember 18, 2022Gupta SC, Ghobbeh A, Taugher-Hebl RJ, Fan R, Hardie JB, LaLumiere RT, Wemmie JA.polyclonal antibody (Millipore, AB1504) diluted 1:1000, goat anti-CA4 polyclonal antibody (R&D Systems, AF2414) diluted 1:1000, mouse anti-PSD95 monoclonal antibody (clone K28/43, catalog no. 05-494, Upstate) diluted

(6) Oral Sensory Neurons of the Geniculate Ganglion That Express Tyrosine Hydroxylase Comprise a Subpopulation That Contacts Type II and Type III Taste Bud CellseneuroOctober 10, 2022Tao Tang, Brian A. Pierchala(βIII-tubulin; 1:200 dilution; Sigma, catalog #T8578), goat α-CAR4 (1:200 dilution; R&D Systems, catalog #AF2414), and rabbit α-TRPM5 (1:200 dilution; Emily Liman’s Lab, University of Southern California). After incubation

(7) Lipopolysaccharide-induced inflammation increases nitric oxide production in taste budsBrain, behavior, and immunityJuly 1, 2022Wu Z, Huang Y, Hu W, Ren L, Jiang P, Margolskee RF, Wang H, Feng S.(Santa Cruz, CA). An affinity-purified goat polyclonal antibody against carbonic anhydrase 4 (CA4, AF2414) was purchased from R&D Systems. An affinity-purified rabbit polyclonal antibody against P2X3 was purchased

(8) Onset of taste bud cell renewal starts at birth and coincides with a shift in SHH functioneLifeMay 19, 2021Erin J Golden, Eric D Larson, Lauren A Shechtman, G Devon Trahan, Dany Gaillard, Timothy J Fellin, Jennifer K Scott, Kenneth L Jones, Linda A BarlowSanta Cruz, sc-206); rabbit anti-NCAM (1:1000; Millipore, AB5032); goat anti-CAR4 (1:1000; R and D Systems, AF2414); rabbit anti-5HT (1:1000; Immunostar, 20080); rabbit anti-SOX2 (1:100; Cell Signaling, 23064); rabbit

(9) Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem CellsJournal of Visualized ExperimentsApril 19, 2021Lauren A. Shechtman, Christina M. Piarowski, Jennifer K. Scott, Erin J. Golden, Dany Gaillard, Linda A. Barlow(for immunohistochemistry),Invitrogen,"D3571, RRID:AB_2307445",1:10000 Goat anti-CAR4,R&D Systems,"AF2414, RRID:AB_2070332",1:50 Guinea pig anti-KRT13,Acris Antibodies,"BP5076, RRID:AB_979608",1:250 Rabbit

(10) Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste BudsJournal of Visualized ExperimentsMarch 1, 2021Lisa C. Ohman, Robin F. Krimmslides,Fisher Scientific (Superfrost Plus Miscroscope Slides),12-550-15, Goat anti-Car4,R&D Systems,AF2414,(1:500) Imaris,Bitplane,pixel-based image analysis software, Neurolucida 360 + Explorer,MBF Biosciences,3D

(11) Selective Peripheral Taste Dysfunction in APP/PS1 Mutant Transgenic MiceJournal of Alzheimer's DiseaseOctober 30, 2020Ryan M. Wood, Zacnite Garcia, Nathan Daniels, Shannon M. Landon, Saima Humayun, Hyoung-gon Lee, Lindsey J. MacphersonVector Laboratories). Primary antibodies used were: anti-TRPM5 (Zuker Lab, 1:500 dilution [15]), anti-Car4 (R&D Systems AF2414, 1:200 dilution), anti-P2X3 (Neuromics GP10108, 1:1000) anti-Aβ (82E1, IBL, 1:500 dilution). Secondary

(12) Optogenetic Stimulation of Type I GAD65⁺ Cells in Taste Buds Activates Gustatory Neurons and Drives Appetitive Licking Behavior in Sodium-Depleted MiceThe Journal of neuroscience : the official journal of the Society for NeuroscienceOctober 7, 2020Baumer-Harrison C, Raymond MA, Myers TA, Sussman KM, Rynberg ST, Ugartechea AP, Lauterbach D, Mast TG, Breza JM.and goat anti-carbonic anhydrase IV/CA4 antigen affinity-purified polyclonal antibody (CA4; catalog #AF2414, R&D Systems). NTPDase2 is a marker for type I cells (Bartel et al., 2006; Dvoryanchikov et al., 2011),

(13) Expression of protocadherin-20 in mouse taste budsScientific reportsFebruary 6, 2020Hirose F, Takai S, Takahashi I, Shigemura N.anti-Gαgust(I-20), cat. no. sc-395, Santa Cruz Biotechnology) or Car4 (1:100; goat anti-CA4, cat. no. AF2414, R&D Systems, Minneapolis, MN, USA) in blocking reagent overnight at 4 °C. The sections were washed

(14) Chemical receptors of the arytenoid: A comparison of human and mouseThe LaryngoscopeFebruary 1, 2020Jetté ME, Clary MS, Prager JD, Finger TE.1:500",Alexa 488; Alexa 647,Donkey anti-goat Carbonic anhydrase 4 (CA4),Type III cells,"R&D Systems, AF2414, AB_2070332","Goat, 1:500",Alexa 488,Donkey anti-goat PGP9.5 (UCHL-1),Neurons and nerve fibers; subpopulation

(15) Visualization of Mouse Choroidal and Retinal Vasculature Using Fluorescent Tomato Lectin PerfusionTranslational Vision Science & TechnologyJanuary 29, 2020Chunhua Jiao, Kelsey Adler, Xiuying Liu, Weize Sun, Robert F. Mullins, Elliott H. Sohnantibodies directed against carbonic anhydrase IV (CA4, R&D Systems, Minneapolis, MN; catalog number AF2414, goat anti-mouse IgG) were applied to sections at a concentration of 2 µg/mL in 0.1% Triton + 0.1% bovine

(16) The Role of the Anion in Salt (NaCl) Detection by Mouse Taste BudsThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 7, 2019Roebber JK, Roper SD, Chaudhari N.with rabbit anti-PLC␤2 (1:1000, Santa Cruz Biotechnology, SC206), goat anti-Car4 (1:500, R&D Systems, AF2414), and chicken anti-GFP (1:1000, Aves, 1020) overnight, followed by fluorescent secondary antibodies

(17) TrkB expression and dependence divides gustatory neurons into three subpopulationsNeural developmentJanuary 28, 2019Rios-Pilier J, Krimm RF.anti-dsRed (1:500, Clontech Laboratories, Inc., Cat No. 632496), and either goat anti-Car4 (1:500, Cat No. AF2414, R&D Systems, Minneapolis, MN). Floating sections were rinsed in 0.1 M PB four times for 15 min and

(18) Effects of insulin signaling on mouse taste cell proliferationPloS oneJanuary 1, 2019Takai S, Watanabe Y, Sanematsu K, Yoshida R, Margolskee RF, Jiang P, Atsuta I, Koyano K, Ninomiya Y, Shigemura N.anti-T1R3, cat. no. sc-22458, Santa Cruz Biotechnology, TX, USA), anti-CA4 (1:100; goat anti-CA4, cat. no. AF2414, R&D Systems, MN, USA), anti-IR (1:100; rabbit anti-IR, cat. no. ab203746, Abcam, Cambridge, UK), anti-Lgr5

(19) Rewiring the taste systemNatureAugust 17, 2017Lee H, Macpherson LJ, Parada CA, Zuker CS, Ryba NJP.dilution), anti-T1R3 (Santa Cruz Biotechnology #sc-22458; 1:500 dilution), anti-Car4 (R&D Systems #AF2414; 1:500 dilution), anti-Plcβ2 (Santa Cruz Biotechnology #sc-206; 1:1000 dilution), anti-GFP (Abcam #ab13970;

(20) The cellular mechanism for water detection in the mammalian taste systemNature neuroscienceJuly 1, 2017Zocchi D, Wennemuth G, Oka Y.

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Carbonic Anhydrase IV/CA4 Antibody from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Carbonic Anhydrase IV/CA4 Antibody from R&D Systems, a Bio-Techne Brand

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