Anti-gamma H2A.X (phospho S139) antibody [9F3] ab26350 from Abcam

Product Specs
ItemAnti-gamma H2A.X (phospho S139) antibody [9F3]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab26350
Antibody TypePrimary
ClonalityMonoclonal
IsotypeIgG
Clone9F3
HostMouse, Nude mouse
Quantity100 UG
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
FormatThis antibody was purified using affinity chromatography
Concentration1 mg/ml
ModificationsPhospho-S139
Research AreaEpigenetics
Gene Nameh2afx
ReactivityChinese hamster, Human, Mouse, Rat
Targetgamma H2A.X

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Acute irradiation induces a senescence-like chromatin structure in mammalian oocytesCommun BiolDecember 12, 2023Claudia Baumann, Xiangyu Zhang, Muthugapatti K. Kandasamy, Xiaohan Mei, Shiyou Chen, Kayvan F. Tehrani, Luke J. Mortensen, Wendy Watford, Ashley Lall, Rabindranath De La Fuenteimmunolabeled with specific antibodies against the DNA double-strand break marker γH2AX (1:400, cat# ab26350, Abcam, Cambridge, USA), and the heterochromatin marker ATRX (1:200, cat# sc15408, Santa Cruz Biotechnology Inc

(2) Microtubule-Targeting Combined with HDAC Inhibition Is a Novel Therapeutic Strategy for Diffuse Intrinsic Pontine GliomasMolecular Cancer TherapeuticsDecember 1, 2023Anahid Ehteda, Aaminah Khan, Gayathiri Rajakumar, Anne S. Vanniasinghe, Anjana Gopalakrishnan, Jie Liu, Maria Tsoli, David S. Zieglerantibodies diluted in 5% BSA in PBST containing overnight at 4°C [anti-gamma H2A.X (1:1,000; Abcam, #ab26350), p21 Waf1/Cip1 (1:1,000; Cell Signaling Technology, #2946), cleaved caspase-3 (1:1,000; Cell Signaling Technology

(3) The Molecular Basis for Selectivity of the Cytotoxic Response of Lung Adenocarcinoma Cells to Cold Atmospheric PlasmaBiomoleculesNovember 20, 2023Mikhail Biryukov, Dmitriy Semenov, Nadezhda Kryachkova, Alina Polyakova, Ekaterina Patrakova, Olga Troitskaya, Elena Milakhina, Julia Poletaeva, Pavel Gugin, Elena Ryabchikova, et al.antibodies: anti-GPX4 (ab125066); anti-GPX7 (ab96257); anti-ATF3 (ab233797), anti-GADD45B (ab205252), γH2AFX (ab26350) and MDM2 (ab16895) (Abcam, Cambridge, UK), anti-β-Tubulin (T8328, Sigma-Aldrich, St. Louis, MO, USA);

(4) Targeting the COMMD4–H2B protein complex in lung cancerBritish Journal of CancerNovember 1, 2023Ming Tang, Joshua T. Burgess, Mark Fisher, Didier Boucher, Emma Bolderson, Neha S. Gandhi, Kenneth J. O’Byrne, Derek J. Richard, Amila Suraweera(Abcam, ab115169), anti-β-actin (BD Biosciences, 612656), anti-FLAG (Sigma, F1804), anti-γH2AX (Abcam, ab26350), anti-H2B (Abcam, ab1790), anti-MDC1 (Abcam, ab11169) and anti-emerin (Cell Signaling 30853). For in vitro

(5) Contributions of viral oncogenes of HPV-18 and hypoxia to oxidative stress and genetic damage in human keratinocytesScientific ReportsOctober 18, 2023Jimena Hochmann, Magdalena Millán, Paola Hernández, Laura Lafon-Hughes, Natali D’ Aiuto, Alejandro Silva, Juan Llaguno, Julia Alonso, Ariel Fernández, Vanesa Pereira-Prado, et al.permeabilized cells were incubated with the anti-γH2AX antibody diluted in blocking buffer (1/500, ab26350; Abcam, Cambridge, Massachusetts, USA) overnight at 4 °C. Finally, cells were washed with PBS three

(6) UBC13-mediated template switching promotes replication stress resistance in FBH1-deficient cellsbioRxiv : the preprint server for biologySeptember 19, 2023Hawks AL, Bergmann A, McCraw TJ, Mason JM.1:1000), Rabbit anti-PCNA (Abcam, Ab18197, 1:1000), and Mouse anti-gamma H2AX (phospho S139) [9F3] (Abcam, Ab26350, 1:1000). TUNEL Assay TUNEL assay performed using Click-IT Plus TUNEL assay kit (Invitrogen) following

(7) The fructose-bisphosphate, Aldolase A (ALDOA), facilitates DNA-PKcs and ATM kinase activity to regulate DNA double-strand break repairScientific reportsSeptember 13, 2023Sobanski T, Suraweera A, Burgess JT, Richard I, Cheong CM, Dave K, Rose M, Adams MN, O''Byrne KJ, Richard DJ, et al.Cell Signaling Technology, for WB 1:1000), Anti-P-ATM S1981 (ab36810, Abcam for IF: 1:200) anti-γH2AX (ab26350, Abcam, for WB and IF 1:1000), anti-p-DNA-PK S2056 (ab124918, Abcam, for WB 1:1000), anti-DNA-PKcs (12311,

(8) HLA Class I Expression Is Associated with DNA Damage and Immune Cell Infiltration into Dysplastic and Neoplastic Lesions in Ulcerative ColitisInternational Journal of Molecular SciencesSeptember 4, 2023Haruka Okami, Naoya Ozawa, Makoto Sohda, Takehiko Yokobori, Katsuya Osone, Bilguun Erkhem-Ochir, Gendensuren Dorjkhorloo, Takuya Shiraishi, Takuhisa Okada, Akihiko Sano, et al.1:100 dilution), anti-PD-L1 (Abcam, ab205921, 28-8, Rabbit mAb, 1:200 dilution), anti-γH2AX (Abcam, ab26350, 9F3, mouse mAb, 1:200), anti-IRF-1 (Abcam, ab186384, Rabbit mAb, 1:300). The Histofine Simple Stain

(9) TAX1BP1 contributes to deoxypodophyllotoxin-induced glioma cell parthanatos via inducing nuclear translocation of AIF by activation of mitochondrial respiratory chain complex IActa pharmacologica SinicaSeptember 1, 2023Wang XZ, Liang SP, Chen X, Wang ZC, Li C, Feng CS, Lu S, He C, Wang YB, Chi GF, et al.(ab32516), GPX4 (ab125066), catalase (ab76024), H2AX (ab229914), TOMM20 (ab186735), phospho-H2AX at Ser139 (ab26350) and phospho-ATM at Ser1981 (ab81292) were all from Abcam company (Cambridge, UK), against PAR (#83732)

(10) Recruitment of the Histone Variant MacroH2A1 to the Pericentric Region Occurs upon Chromatin Relaxation and Is Responsible for Major Satellite Transcriptional RegulationCellsAugust 30, 2023Ludmila Recoules, Nicolas Tanguy Le Gac, Fatima Moutahir, Kerstin Bystricky, Anne-Claire LavigneAntibody,Supplier (Reference),Dilution (Use) * HIRA,Cell signaling (D2A5E),1/1000 (WB) γH2AX,Abcam Ab26350 [9F3],1/1000 (WB & IF) γH2AX,Abcam Ab81299,1/1000 (IF) mH2A1.2,Millipore #MABE61 Clone 14GT,1/1000 mH2A1,Millipore

(11) Long noncoding RNA BBOX1-AS1 increased radiotherapy sensitivity in colorectal cancer by stabilizing and activating PFK1Transl OncolAugust 4, 2023Qi Wang, Xiao-Fei Li, Ying-Hui Zhou, Xiang-Hong Qin, Li-Hui Wang, Meng-Qing Xiao, Ke Cao, John K. Ma, Cheng-Hui HuangCat:13,347-2-AP); anti-LDHA (ptgcn, Cat:21,799-1-AP); anti-GLUT1 (ptgcn, Cat:21,829-1-AP); anti-γ-H2AX (Abcam, Cat: ab26350); and anti-β-actin (ptgcn, Cat:66,009-1-Ig). Cell migration assay Colo205 and HCT116 cells were inoculated

(12) MAGI1 Prevents Senescence and Promotes the DNA Damage Response in ER+ Breast CancerCellsJuly 25, 2023Janine Wörthmüller, Simona Disler, Sylvain Pradervand, François Richard, Lisa Haerri, Gustavo A. Ruiz Buendía, Nadine Fournier, Christine Desmedt, Curzio Rüegganti-GAPDH (1:5′000; Cat. No. G9545, Sigma-Aldrich), anti-gamma H2A.X (phospho Ser139) (1:1′000, Cat. No. ab26350, Abcam, Cambridge, UK), anti-AKT (Cat. No. 9272), anti-phospho AKT (S473) (Cat. No. 4060), anti-p44/42

(13) FIBP interacts with transcription factor STAT3 to induce EME1 expression and drive radioresistance in lung adenocarcinomaInternational Journal of Biological SciencesJuly 24, 2023Yunhong Xu, Jun Li, Kuikui Zhu, Yulan Zeng, Jing Chen, Xiaorong Dong, Sheng Zhang, Shuangbing Xu, Gang WuCA, USA). Antibodies The anti-FIBP (ab236647), anti-Rad51 (ab133534) and anti-γH2AX (phospho S139) (ab26350) antibodies were purchased from Abcam (Cambridge, MA, UK). The anti-GST (#2624), anti-phospho-STAT3 (Ser727

(14) Proper RPA acetylation promotes accurate DNA replication and repairNucleic acids researchJune 23, 2023Gan X, Zhang Y, Jiang D, Shi J, Zhao H, Xie C, Wang Y, Xu J, Zhang X, Cai G, et al.extra 15 min. After being blocked with 5% BSA, cells on coverslips were incubated with the anti-γH2AX (ab26350, Abcam), anti-RPA1(ab176467, Abcam) or anti-RAD51(ab133534, Abcam) antibody at 4°C overnight. Following

(15) Activity and Selectivity of Novel Chemical Metallic Complexes with Potential Anticancer Effects on Melanoma CellsMoleculesJune 19, 2023Maria Camilla Ciardulli, Annaluisa Mariconda, Marco Sirignano, Erwin Pavel Lamparelli, Raffaele Longo, Pasqualina Scala, Raffaella D’Auria, Antonietta Santoro, Liberata Guadagno, Giovanna Della Porta, et al.(sc43765) and anti-GAPDH (sc365062), provided from Santa Cruz Biotechnology, and anti-phospho H2AX (ab26350 Abcam). Immunoreactivity was detected by sequential incubation with appropriate horseradish peroxidase-conjugated

(16) Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virusMolecular Therapy - Nucleic AcidsJune 13, 2023Dmitry Kostyushev, Sergey Brezgin, Anastasiya Kostyusheva, Natalia Ponomareva, Ekaterina Bayurova, Natalia Zakirova, Alla Kondrashova, Irina Goptar, Anastasiya Nikiforova, Anna Sudina, et al.anti-AID (ab59361) antibodies, or with rabbit anti-53BP1 antibodies (ab21083), mouse γ-H2AX antibodies (ab26350), or rabbit anti-H2AX antibodies (ab11175) at 4°C overnight with gentle shaking. Samples were then washed

(17) Effects of Cyanobacterial Harmful Algal Bloom Toxin Microcystin-LR on Gonadotropin-Dependent Ovarian Follicle Maturation and Ovulation in MiceEnvironmental health perspectivesJune 1, 2023Wang Y, Pattarawat P, Zhang J, Kim E, Zhang D, Fang M, Jannaman EA, Yuan Y, Chatterjee S, Kim JJ, et al.(1:1,000, ab6276; Abcam), anti-PCNA (1:1,000, ab2426; Abcam), anti-γH2AX (phospho Ser139) (1:1,000, ab26350; Abcam), anti-FOXO1A (phospho S253) (1:1,000, ab259337), anti-AKT (1:1,000, 9272S; Cell Signaling Technology),

(18) The FBXO32/ATR/ATM axis acts as a molecular switch to control the sensitivity of osteosarcoma cells to irradiation through its regulation of EXO1 expressionActa biochimica et biophysica SinicaMay 24, 2023Lu Y, Huang P, Li Y, Liu W, Li J, Zhao R, Feng H, Shi C, Cao G.CST, Danvers, USA), p-ATR (#30632S; CST), p-CHK1 (#12302; CST), FBXO32 (ab168372; Abcam), γ-H2A.X (ab26350; Abcam), HA (AF5057; Beyotime), Flag (AF5051; Beyotime), β-Actin (#2D4H5; Proteintech, Chicago, USA),

(19) circCIMT Silencing Promotes Cadmium‐Induced Malignant Transformation of Lung Epithelial Cells Through the DNA Base Excision Repair PathwayAdvanced ScienceMay 1, 2023Meizhen Li, Wei Chen, Jinjin Cui, Qiuyi Lin, Yufei Liu, Huixian Zeng, Qiuhan Hua, Yihui Ling, Xiaodi Qin, Yindai Zhang, et al.were incubated at 4 °C with primary antibodies. The following antibodies were used: γ‐H2AX (1:1000, ab26350, Abcam), APEX1 (1:1000, ab268072, Abcam), XRCCI (1:1000, ab134056, Abcam), PARP1 (1:1000, ab191217,

(20) Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesisAsian journal of andrologyMay 1, 2023Wang XL, Li JM, Yuan SQ.for WB, 1:200 for IMF) were purchased from ABclonal Technology (Wuhan, China). Anti-γ-H2A.X antibody (ab26350, 1:200 for IMF) was purchased from Abcam (Shanghai, China). Secondary antibodies specific to HRP goat

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Anti-gamma H2A.X (phospho S139) antibody [9F3] from Abcam

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Anti-gamma H2A.X (phospho S139) antibody [9F3] from Abcam

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