Fig 1: NLC drive expression of NTSR2-TrkB and pro-survival signaling in B-CLL cells. (a) Brightfield microscopy of co-cultured NLC (red arrows) and B-CLL cells (blue arrows). (b) Confocal microscopy analysis of CD14 (green) and CD68 (red) (left panel), and CD163 (green) and CD68 (red) (right panel), expression by NLC (n = 3). Nuclei are stained with DAPI. (c) Western blot analysis of CD68, CD163, and CD206 expression by NLC (n = 3). (d) Representative western blots showing expression of NTSR2, TrkB, p-Src, Src, and Bcl-2 by B-CLL cells isolated from two independent patients and cultured alone or with autologous NLC for 48 h. (e) Quantification of NTSR2 (n = 15), TrkB (n = 6), p-Src/Src (n = 17) and Bcl2 (n = 16) expression in independent patient samples. Data are presented as the mean ± SEM from at least three independent experiments (*p < 0.05 and **p < 0.01). Blots are cropped for clarity; full-length blots are shown in the Supplementary Fig. S7.