Fig 1: Effects of low‐dose lithium supplementation on soleus and extensor digitorum longus (EDL) NFAT activation, PGC‐1α and MHC isoform expression. (a) Representative Western blot images of phosphorylated and total nuclear factor activated T cells (p‐NFATc1 and NFATc1) and peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha (PGC1‐α), all of which are downstream markers of calcineurin signaling. For pNFATc1, NFATc1, and PGC‐1α, 7.5 μg of total protein was loaded into each well. (b) Quantification of p‐NFATc1 and NFATC1 were first normalized to ponceau prior to calculating the p‐NFATc1/NFATc1 ratio. (c) Quantification of PGC1‐α content in soleus and EDL muscles in control‐fed and LiCl groups normalized to ponceau. (d) Representative Western blot images of MHC I and IIa in soleus muscles from control and lithium‐fed mice. For both isoforms, 5 μg of total protein was loaded into each well. Quantification of MHC I (e) and MHC IIa (f) normalized to ponceau. For (b,c) and (e,f), all data are expressed relative to control. *p < .05, significantly different from control, using a Student's t tests (n = 6–9 per group). All data are expressed as means ± SD