Fig 1: Differential expression of Mettl1 and Wdr4 during neurogenesis. (A, B) Immunofluorescence staining for Mettl1 and Wdr4 in Nestin + NSCs, Tuj1 + neurons and GFAP + astrocytes. Scale bar, 150 μm. (C, D) Western blotting and quantification for Mettl1 in NSCs, neurons and astrocytes. n = 3, ***P < 0.001 compared with NSCs. (E, F) Western blot and quantification for Wdr4 in NSCs, neurons and astrocytes. n = 3, ***P < 0.001, *P < 0.05 compared with NSCs. (G, H) The expressions of Mettl1 and Wdr4 mRNA in NSCs, neurons and astrocytes. n = 3, ***P < 0.001 compared with NSCs. (I) Immunofluorescence staining for Mettl1 costaining with Nestin, NeuN and GFAP in dentate gyrus of hippocampus. Scale bar, 200 μm. Data are represented as the mean ± SEM. NSCs, neural stem cells; n represents number of independent experiments