Fig 1: Data-independent acquisition mass spectrometry analysis and WB detection of SPOP and NF-?B expression with SERPINA3 overexpressing lung cancer cells and tumor tissues. (A) Flow chart for protein filtration. (B) Volcano diagram of DEPs analysis. (C) Dot-bubble plots of Gene Ontology annotation analysis. (D) The DEPs involved in 18 functions were analyzed by heat map. (E) Analysis of the protein-protein interaction analysis using Cytoscape software. (F) Dot-bubble plots of the protein pathway enrichment analysis. (G) Survival analysis of SPOP in lung cancer with Kaplan-Meier plotter database. (H) The Gene Expression Profiling Interactive Analysis database was used to analyze the correlation between SPOP and SERPINA3 expression. (I) SPOP and NF-kB p65 expression levels were determined by WB in lung cancer cells. (J) Detection of SPOP and NF-kB p65 expression levels in tumor tissues by WB. (K) Quantification analysis of protein expression levels. Data were expressed as the mean ± SD. *P<0.05. WB, western blotting; SPOP, speckle-type POZ protein; SERPINA3, serpin family A member 3; DEPs, differentially expressed proteins.