Fig 1: The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression (n = 6 mice/group, *p < 0.05, **p < 0.01, unpaired t-test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of PSD95 and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice (n = 4 mice/group, *p < 0.05, unpaired t-test).
Fig 2: Interaction networks analyzed for differentially expressed proteins (DEPs). (A) STRING protein–protein interaction network of DEPs colored nodes indicates the individual protein identified. Lines between nodes represent the direct and indirect association of proteins. Red color presented up-regulated proteins. Green color presented down-regulated proteins. Circle 1: synaptic interaction proteins such as CPLX1, SNAP25, and SYT1 proteins. Circle 2: mitochondrial interaction proteins such as ALDH1B and GATM. (B) Heatplot presents differentially expressed proteins related to the pathway. Red ovals indicated proteins such as CPLX1, SNAP25, and SYT1 involved in the synaptic vesicle cycle pathway, and ALDH1B and GATM involved in arginine and proline metabolism.
Fig 3: Validation of synaptic- and mitochondrial-related proteins using immunofluorescence and Western blot assay. (A,B) Representative immunofluorescence staining images (A) and quantitative analysis (B) for SNAP25, GATM, and NDUFA11 in the spinal cord. Scale bar = 20 µm. Data were expressed as the mean ± SD (n = 3 mice/group). *p < 0.05 vs. sham group. (C,D) Representative Western blot bands (C) and quantitative analysis (D) of CPLX1, SNAP25, and SYT1 protein in the spinal cord. Data were presented as mean ± SD (n = 3 mice/group). *p < 0.05 vs. sham group. (E,F) Representative western blot bands (E) and quantitative analysis (F) of ALDH1B, GATM, and NDUFA11 protein in spinal cord. Data were presented as mean ± SD (n = 3 mice/group). *p < 0.05 vs. sham group.
Supplier Page from ABclonal Technology for SNAP25 Rabbit mAb