Fig 2: LTP induced CALU-1 cell death via VEGF/VEGFR2/RAS/ERK axis. (a) The expressions of key proteins in this signaling pathway were assessed by Western Blot analysis, (b) the data were quantitated and graphed. Data represent the means ± SD of three independent experiments, compared with 0 s LTP treatment time group. * p < 0.05; *** p < 0.001; compared with the 0 s LTP treatment group.

Fig 3: OGDHL inhibits ccRCC progression by inhibiting FASN-regulated lipid metabolism and ERK signaling pathways.A Volcano plot of whole transcriptome sequencing data after OGDHL overexpression. FASN was a significantly downregulated gene after OGDHL overexpression. B. Protein and mRNA levels of FASN after stable overexpression of OGDHL were detected by western blot analysis and qRT-PCR; t-test, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 (independent samples t-test). C Protein and mRNA levels of FASN after OGDHL knockdown were detected by western blot analysis and qRT-PCR; t-test, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 (independent samples t-test). D The GSEA correlation charts are based on the TCGA-KIRC database with FASN expression as the premise for screening. FDR < 25% and p < 0.05 was considered statistically significant. E Expression of key members of the MAPK signaling pathway was detected in FASN knockdown and control ccRCC cell lines by western blotting. F Expression of key members of the MAPK signaling pathway was detected in FASN-overexpressing and control ccRCC cell lines by western blotting. G Western blot analysis was used to detect the protein levels of OGDHL and FASN in the established stable cell lines. H Transwell assay results for migration and invasion of stable cell lines; t-test, ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 (independent-samples t-test for statistics). I Western blotting detected the expression of key members of MAPK signaling pathway in ccRCC functionally restored cell lines constructed by overexpressing OGDHL and overexpressing FASN lentivirus. J Oil red O staining photograph of ccRCC cells.