Fig 1: The protective effects of pseudorabies virus XJ delgE/gI/TK in mice brain. (A) Histopathological observations of mice brain injected with the indicated viral strain or Dulbecco’s modified Eagle’s medium. The arrows from the left to the right show nuclear cleavage, neuron phagocytosis, and vacuolar neuronal degeneration in the brain, respectively (hematoxylin and eosin staining, × 200 magnification). (B) Immunohistochemistry detection of SYN1 in mice. Comparison of the SYN1 expression in mice brain using immunohistochemistry. (C) Western blot analysis and relative quantification of the band density of SYN1. (D) The fold change of TNF-a and IL-6 in the brain was determined by qRT-PCR. (E) The fold change of TNF-a and IL-6 in the intestine was determined by qRT-PCR. (F) The fold change of IFN-ß, IFN-?, ISG15, Mx1, and OAS1 in the brain was determined by qRT-PCR. (G) The fold change of IFN-ß, IFN-?, ISG15, Mx1, and OAS1 in the intestine was determined by qRT-PCR. Data are presented as mean ± SD (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.