Fig 2: JMJD1C knockdown prevents glycolysis in mice exposed to hypoxia.A The mRNA levels of HK2, PGK1 and LDHA in lung tissues were measured by qPCR. B Western blot analysis of HK2, PGK1 and LDHA protein expression in lung tissues. C Serum lactate levels were measured. D The protein levels of p-STAT3 and STAT3 were examined using Western blotting. Nor Normoxia, Hx Hypoxia. The values are the mean ± SD, and statistical significance was determined by one-way ANOVA. N = 6, **p < 0.01.

Fig 3: JMJD1C knockdown prevents proliferation and glycolysis in PASMCs exposed to hypoxia.A The survival of PASMCs was assessed by CCK8 assays. B Representative immunofluorescence images of PASMCs stained with anti-Ki67 antibodies (red). Cell nuclei were stained with DAPI (blue). Bar = 50 μm. C Western blot analysis of Cyclin D1 and CDK4 protein levels in PASMCs. D The mRNA levels of HK2, PGK1, and LDHA in PASMCs were determined by qPCR. E Western blot analysis of HK2, PGK1, and LDHA protein levels in PASMCs. F Cell supernatants were used to assess lactate levels. Nor Normoxia, Hx Hypoxia. The values are the mean ± SD, and statistical significance was determined by one-way ANOVA. N = 3, *p < 0.05, **p < 0.01.