Fig 2: (A) Quantitative analysis results and representative images of the Western blot results for MMP2 and MMP9 in ALT1 knockdown HepG2 cells. (B) Quantitative analysis results and representative images of the Western blot results for MMP2 and MMP9 in EP-CAM knockdown HepG2 cells. (C) RT-qPCR analyses showed the knockdown of ALT1 in HepG2 cells significantly decreased expression of N-cadherin, Snail, and Twist while increasing E-cadherin expression. (D) RT-qPCR analyses showed the knockdown of EP-CAM in HepG2 cells significantly decreased expression of N-cadherin, Snail, and Twist while increasing E-cadherin expression.

Fig 3: BAF-L does not affect the early progress of mouse spermatogenesis. (A) Representative images of H&E staining show normal histological structure of BAF-L KO testis at the age of P10, P14, and P21. (B,F) Representative images of GATA-4 labeling and quantification show comparable Sertoli and Leydig cells in BAF-L KO testis at the age of P10 (n = 4), P14 (n = 3), and P21 (n = 3). Error bars indicate SD. Red arrows indicate Sertoli cells, and green arrows indicate Leydig cells. (C,G) Representative images of EpCAM labeling and quantification show comparable spermatogonia cells in BAF-L KO testis at the age of P10 (WT, n = 4; KO, n = 5), P14 (n = 4), and P21 (WT, n = 4; KO, n = 5). Error bars indicate SD. Arrows indicate spermatogonia cells. (D,H) Representative images of SCP3 labeling and quantification show comparable spermatocytes in BAF-L KO testis at the age of P10 (WT, n = 3; KO, n = 4), P14 (n = 3) and P21 (n = 3). Error bars indicate SD. Arrows indicate spermatocytes. (E) Quantification shows no difference in the ratio of testis and bodyweight of BAF-L KO mice at the age of P10 (WT, n = 7; KO, n = 6), P14 (WT, n = 4; KO, n = 5) and P21 (WT, n = 8; KO, n = 6). Error bars indicate SD.