Fig 1: MO improves GluN2B phosphorylation by decreasing STEP in APP/PS1 mice. (A) The expression level of GluN1, GluN2A, GluN2B, and p-Y1472 from hippocampal lysates of WT and APP/PS1 mice with or without MO treatment was evaluated by Western blotting. (B–E) The statistical analysis of Western blot bands from panel A. (F) The expression level of STEP, np-S221, Fyn, and p-Y416 from hippocampal lysates of WT control and APP/PS1 mice with or without MO treatment was examined. (G–J) The statistical analysis of Western blot bands from panel F. ß-actin serves as the loading control. The data are presented as Mean ± SEM, (n = 3). ** p < 0.01 and *** p < 0.001 vs. WT or vs. APP/PS1 mice groups. p-Y1472 = phosphorylated GluN2B at Tyr1472; np-S221 = non-phosphorylated STEP at Ser221; p-Y416 = phosphorylated Fyn at Tyr416.