Fig 1: CeA treatment attenuated mesenchymal transition with downregulation of FSP-1 and snail mRNA expression. (A) Immunotaining of fibroblast (PDGFR-ß) and myofibroblast (a-SMA). Fibroblast were stained in interstitial areas of SO group, meanwhile fibroblast expansion occurred in UUO as shown by expansion of positive staining in interstitial areas (white arrows). Positive staining of a-SMA showed smooth muscle cells of vessels (black arrows) in SO, meanwhile a-SMA revealed myofibroblast in interstitial areas of UUO group (grey arrows). (B) Double immunofluorescence staining demonstrated colocalization of fibroblast (red) and myofibroblast (green) which revealed fibroblast to myofibroblast transition. (C,D) Representative picture and semiquantitative analysis of RT-PCR analysis showed Snail and FSP-1 mRNA expressions.