Fig 1: Western blot detection of MOBP in cerebellar white matter lysates (N = 29). Immunoblots were labelled with anti-MOBP (Atlas Antibodies HPA035152, 1:250), anti-ß-actin (Sigma A1978, 1:5000) and IRDye secondary antibodies (LiCor), and scanned on a LiCor Odyssey Fc (C). Intensities for the 20.8–23.3 kDa (isoforms b and a) and 9.5 kDa (isoform c) bands, respectively, were derived from raw scan data, analysed on Image Studio (LiCor), and normalized against ß-actin band for each sample as housekeeping gene (A, B). Analysis of the ratio between levels of 20.8–23.3 kDa (isoforms b and a) over 9.5 kDa (isoform c) bands (D). *Horizontal black bars indicate significant differences (adjusted p < 0.05 using Wilcoxon rank-sum test with Benjamini–Hochberg adjustment for multiple testing). CTRL, healthy controls; MSA, multiple system atrophy; PD, Parkinson's disease; PSP, progressive supranuclear palsy; HD, Huntington's disease.
Supplier Page from MilliporeSigma for Anti-MOBP antibody produced in rabbit