Fig 2: MicroRNA-152 downregulated Bcl-2 and NF-?B in A549/cis cells. (A) A549/cis cells were transfected with microRNA-152 mimics, and Bcl-2 mRNA levels were detected via RT-qPCR analysis. (B) A549/cis cells were transfected with microRNA-152 mimics, and NF-?B mRNA levels were determined via RT-qPCR analysis. (C) Western blotting. Changes in Bcl-2 and NF-?B protein expression levels in A549/cis cells induced by microRNA-152 mimics. A549/cis cells, cisplatin-resistant A549 cells; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control.

Fig 3: Ant34a upregulates cardiac expression of Bcl-2 and SIRT1 in DOXO treated rats. (a) Bcl-2 mRNA and protein levels were evaluated by qPCR and western blot analysis respectively. (b) SIRT1 mRNA and protein levels were evaluated by qPCR and western blot analysis respectively. (c) Representative images of western blot protein bands. Full-length blots are displayed in Supplementary Fig. S1a–b. mRNA and protein expressions are shown as fold change with respect to CTL group. Results are presented as mean ± SD, n = 5–8/group. *p < 0.05 vs CTL; °p < 0.05 vs DOXO; #p < 0.05 vs D + AntCTL. D: DOXO.

Fig 4: Effects of BPA exposure on apoptosis. (a) Western blotting representative bands of Bax and Bcl2. (b) Protein expression of Bax and Bcl2 expressed as ratio at 45 PND and 60 PND. (c–f) Apoptotic cells (green) are shown by TUNEL assay in rat testis at 45 PND (c,d) and 60 PND (e,f). (g) Fraction of tubules with more than three apoptotic cells. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 vs age-matched control group. Scale bar, 50 µm. tub: tubulin; TUNEL: terminal deoxynucleotidyltransferase-mediated dUTP nick end labelling; C: control group: BPA: bisphenol A-exposed group; PND: postnatal day.

Fig 5: Bcl-2 and NF-?B are upregulated in A549/cis cells. (A) The Cell Counting Kit-8 method was used to determine the chemotherapeutic resistance of A549/cis cells. IC50 was calculated to determine the degree of drug resistance of the cells. (B) MicroRNA-152 was downregulated in A549/cis cells. Expression levels of microRNA-152 in A549 cells and transfected A549/cis cells were measured via RT-qPCR analysis. (C) NF-?B expression levels in A549 cells and A549/cis cells were measured RT-qPCR analysis. (D) Bcl-2 expression levels in A549 cells and A549/cis cells were measured via RT-qPCR analysis. (E) Protein bands in the image. (F) Protein band intensity. A549/cis cells, cisplatin-resistant A549 cells; IC50, half maximal inhibitory concentration; RT-qPCR, reverse transcription quantitative PCR.