Fig 1: TAF9B is associated with PCAF.(A) TAF9B and TBP were immunoprecipitated from EB-MN cells differentiated for 8 days and co-immunoprecipitated proteins analyzed by western blotting using antibodies against the TFIID subunits TAF4 and TAF7, and the histone acetyltransferase PCAF. (B) 293T cells were transfected with pCMV-3xFLAG-TAF9B or -TAF9, immunoprecipitated with TAF9B antibodies, and analyzed by western blotting using anti-Flag antibodies. (C) TAF9B was immunoprecipitated from spinal column extracts from WT and Taf9b KO newborn mice and co-immunoprecipitated proteins were analyzed by western blotting detecting TAF4, TBP and PCAF.DOI: http://dx.doi.org/10.7554/eLife.02559.011