Fig 2: Effect of LFMS on glial fibrillary acidic protein (GFAP) level in the SNpc and ST regions of MPTP-induced mice. Representative images of GFAP immunopositive cells in SNpc (A–D) and ST (E–H) regions (at 40× and 100× magnification, respectively) of control (A,E), MPTP (B,F), MPTP + LFMS (C,G), and LFMS alone (D,H) treated mice brain; boxes on the images indicate the region where quantification was performed; and (I) graph representing the percentage number of GFAP immunopositive cells in the SNpc and ST regions. (J) Representative Western blot images of the GFAP and IBA1 levels and their respective loading control in the SNpc and ST regions of the control, MPTP, MPTP + LFMS, and LFMS mice brain. The graph represents the levels of GFAP (K) and IBA1 (L) via Western blotting, n = 6.