Fig 2: Changes in gene expression dependent on Baf250A or Brd9 knockdown. Volcano plots displaying differentially expressed genes between scr control and Baf250A (A) or Brd9 (B) knockdown C2C12 cells at 48 h of differentiation. The y-axis corresponds to the mean log10 expression levels (p values). The red and blue dots represent the up- and downregulated transcripts in knockdown cells (false-discovery rate (FDR) of <0.05), respectively. The gray dots represent the expression levels of transcripts that did not reach statistical significance (FDR of >0.05). (C) Venn diagram showing the overlapping differentially expressed genes between the differentiating myoblasts knocked down for the Baf205A and Brd9 subunits. GO term analysis of differentially expressed genes in differentiating C2C12 cells knocked down for Baf250A (D) or Brd9 (E). Cut-off was set at 2.0 of the -log (adjusted p value). See Table S2 for the complete list of genes.

Fig 3: Baf250A, Brd9, and Baf180 expression in wild-type (WT), shRNA scrambled (scr) control, and the indicated shRNA-mediated knockdowns in differentiating C2C12 myoblasts. Representative Western (top) and quantification (bottom) of Baf250A (A), Brd9 (B), and Baf180 (C) levels in differentiating cells after 48 h of differentiation. Data represents the mean ± SE of three independent biological replicates. GAPDH was used as the loading control. Quantification of each sample was compared to the corresponding wild-type (WT) sample, which was set to 1.0. *** p < 0.001.