Fig 1: MRL MSC produced and released higher amount of MANF than BL6, BALB/c and DBA1 MSC. (A) Proteomic analysis of differential expression of MANF, GAS6, NENF, and SEMA5A in MRL MSC compared to BL6 MSC secretomes. Effect size indicate the standardized mean difference in protein expression level between MRL MSC and BL6 MSC. For each protein, the median intensity levels (Log 2 value) in MRL MSC and BL6 MSC are indicated. Normalized protein intensities were used to calculate the Effect size MRL/BL6. (B) Western blot analysis of MANF, GAS6, NENF, and SEMA5A in whole-cell extracts from BL6, BALB/c and DBA1 MSC. The intensity value of each target protein band was normalized against the intensity value of a-Tubulin gel band used as the internal loading control for each sample. (C) MANF mRNA expression level in MRL MSC and BL6 MSC. Western blot analysis of supernatants of BL6, BALB/c, and DBA1 MSC showing blotted proteins stained with Ponceau S (D) and the MANF protein band revealed by the anti-MANF antibody (E). MRL MSC whole cell extract was used as a positive control.