Fig 1: The uncoupling of glycolysis and mitochondrial respiration favored by MPC2 blockage induce a FK866 resistant-like phenotype in PAR cells. Co-treatment of (A) PAR and (B) RES cells with UK5099 (0.1–100) µM and FK866 as well as with FK866 single administration (Final concentrations of FK866: 5 nM for PAR cells and 20 nM for RES). Viability assessed by OzBlue assay after 48 h of exposure and compared with DMSO condition. Effect of MPC1 (siMPC) and/or MPC2 (siMPC2) transient silencing in PAR cells in terms of (C) cell viability and (D) relative ATP level, in combination with FK866 5 nM. All the experiments were performed in triplicate. Repeated measures one-way ANOVA was used to calculate statistical significance (*P<0.05, **P < 0.01, ***P < 0.001) between control and experimental conditions.