Fig 1: Effects of miR-365b-3p expression on NSCLC cell proliferation, cell cycle distribution and cell apoptosis. A549 and H1299 cells were transfected with miR-365b-3p mimics and inhibitor, respectively, for 48 h. (A) miR-365b-3p expression level was detected using reverse transcription-quantitative PCR. Proliferation of A549 and H1299 cells was determined using (B) Cell Counting Kit-8 assay and (C) colony formation assay. (D and E) Analysis of the percentage of cells in each phase of the cell cycle in A549 and H1299 following various transfections. (F and G) Apoptotic rates of A549 and H1299 cells were determined by flow cytometry. (H) Protein expression of CDK4, cyclin D1, Bax and Bcl-2 measured by western blotting. Data were expressed as the means ± standard deviation of at least three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-NC. NSCLC, non-small cell lung cancer; miR, microRNA; NC, negative control; PI, propidium iodide; FITC, fluorescein isothiocyanate; OD optical density.
Fig 2: Isoflurane restrains proliferation and facilitates apoptosis in CRC cells partly through miR-216. (A–C) The number of clones of SW620 and HCT116 cells treated with isoflurane and/or miR-216 mimic. (D–F) Apoptotic rate of SW620 and HCT116 cells under treatment with isoflurane and/or miR-216 mimic. (G–I) Expression of Caspase3, Bax and Caspase3 and Bax proteins in SW620 and HCT116 cells following treatment with isoflurane and/or miR-216 mimic. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 3: The promotional effects of isoflurane on CRC cell apoptosis. Apoptosis rates of (A,B) SW620 and (C,D) HCT116 cells under treatment with 40 μM isoflurane. (E–G) Expression of Caspase3, Bax and Bcl-2 proteins in SW620 and HCT116 cells following exposure to 40 μM isoflurane. **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 4: Effects of warm acupuncture combined with BMSCs on the expressions of Bax, Bcl-2, and Caspase-3 in cartilage tissue of knee joint. (a) Immunohistochemical results of Bax, Bcl-2, and Caspase-3 for the different groups. (b–d) Quantification of qPCR data of Bax, Bcl-2, and Caspase-3 in cartilage tissue of knee joint. Data are expressed as mean ± SEM (n = 10). ∗∗p < 0.01 versus the blank group, ##p < 0.01 versus the KOA group, and +p < 0.05 versus the warm acupuncture combined with the BMSCs group.
Fig 5: circ_0066147 silencing regulated cell proliferation, migration, invasion and apoptosis in vitro. SW1990 and PANC-1 cells were transfected with si-NC or si-circ_0066147. (a) qRT-PCR for circ_0066147 expression in transfected cells. β-Actin served as the reference gene. (b and c) MTT assay for cell proliferation. (d) Flow cytometry for cell apoptosis. (e) Western blot for PCNA, Bcl-2, Bax and C-caspase3 levels. GAPDH served as the reference gene. (f) Wound-healing assay for cell migration. (g) Transwell assay for cell invasion. *P < 0.05.
Supplier Page from Abcam for Anti-Bax antibody [2D2]