Fig 1: Effect of SDF-1a/CXCR4 signaling on VEGF, bFGF, IL-8 and ICAM-1 expression of HRECs. (A) Protein extracts were obtained and subjected to western blotting. Representative results of VEGF, bFGF, IL-8 and ICAM-1 expression of HRECs were determined. (B) Statistical analysis of ratios of VEGF, bFGF, IL-8 and ICAM-1 to GAPDH protein. All values represent mean ± standard error of the mean (n=6-8 animals). *P<0.05 vs. control. SDF, stromal cell-derived factor; CXCR, chemokine (C-X-C motif) receptor; HRECs, human retinal endothelial cells; bFGF, basic fibroblast growth factor; ICAM, intercellular cell adhesion molecule.
Fig 2: CXCR4 gene and protein expressions in HRECs. (A) Semi-quantitative RT-PCR to evaluate mRNA expression of CXCR4. HRECs were harvested to extract total RNAs. RT-PCR was performed using the obtained total RNAs. (B) Western blotting was used to evaluate protein expression of CXCR4. HRECs were harvested to extract the total protein (n=3). (C) IgG isotype control performed by flow cytometry. (D) Flow cytometry to examine the expression of CD106 and CD54 in HRECs. CXCR, chemokine (C-X-C motif) receptor; HREC, human retinal endothelial cell; RT-PCR, reverse transcription-PCR.
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