Fig 1: Visualization of human cholinergic transmission with iAChSnFR.A Schematic of the differentiation of cholinergic neurons from human-induced pluripotent stem cells (hiPSCs). CAT anti-choline acetyltransferase staining (Abcam, #ab223346), MAP2 anti-microtubule-associated protein 2 staining (Abcam, #ab32454), DAPI 4',6-diamidino-2-phenylindole nucleic acid staining (Sigma-Aldrich, D9542). Note the authentication of hiPSCs in the previous report [67]. B Imaging fluorescence responses of an iAChSnFR expressing human iPSC-derived neuron evoked by a train of 20 stimulating pulses delivered at 32 Hz. C Deconvolution microscopic analysis of three-dimensional spatiotemporal ?F/F profiling of the iAChSnFR expressing human iPSC-derived neuron to local electrical stimuli. Note one isolated release site indicated by pink arrow in C. D Spatial profiling of the isolated release site indicated by the pink arrow in C with a pixel-wise maximal ?F/F plot. Fitting the data points in this plot with a single exponential decay function (pink line) yields an estimated ACh spread length constant of 0.88 µm.
Supplier Page from Abcam for Anti-Choline Acetyltransferase antibody