Fig 1: Identification and validation of hub genes in AS. (A) Relative expression levels of ICAM1, KLF2, and VCAM1 in endothelial cells from four groups. (B) Prediction of transcription factors for ICAM1, KLF2, and VCAM1. (C) Western blot validating the protein expression of ICAM1, KLF2, and VCAM1 in human AS coronary artery and normal tissues. (D) RT-qPCR validating the mRNA expression of ICAM1, KLF2, and VCAM1 in human AS coronary artery and normal tissues. #p < 0.05.
Fig 2: Validation of hub genes in human AS coronary artery tissues. (A) H & E, EVG, and Masson staining results of human AS coronary artery and normal tissues. For H & E staining, the nucleus was stained blue, and the cytoplasm was stained red. For EVG staining, elastic fibers and nuclei were dyed black, and the collagen fibers were dyed red. For Masson staining, collagen fibers and cell nuclei were stained blue, and the muscle fibers and cellulose were stained red. (B,C) Immunofluorescence detecting ICAM1, KLF2, and VCAM1 expression in human AS coronary artery and normal tissues. Scale bar = 200 μm. Magnification: ×4. #p < 0.05.
Supplier Page from Abcam for Anti-KLF2 antibody