Fig 1: Neurospheres maintain the charactors for self-renewable and multipotent NCCs. (A) Primary neurospheres were formed at day 7-8(A,e). At 24 h, cells grew with adherence (A,a, red arrow head). After replating, tiny clusters can been seen at day 2 (A,b, red arrow head), which then became a dome (A,c, red arrow head) and neurospheres on day five (A,d, red arrow head). (B) Flow cytometry was used to detect the percentage of P75+ or CD49d+. The positive rate of P75+/CD49d+ was 5.09%. (C) The percentage of P75+/Nestin+ cells were 6.4%, which was detected by immunofluorescence. (D) Primary neurospheres were differentiated into neuron (TUJ1+), glial cells (GFAP+), and smooth muscle cells (a-SMA+).