Fig 1: (A) mRNA levels of enzymes involved in nucleotide metabolism in mouse liver, Actb was chose as the housekeeping genes to normalize the qPCR results. (B) Protein levels of ADSL, APRT, PNP, and ADA in HepG2 cells after serum shock. (C) Urate levels in cell culture supernatant at different points after serum shock. (D) Per2 mRNA levels in liver after T3 treatment, Actb was chose as the housekeeping genes to normalize the qPCR results. (E) Per2 mRNA levels in HepG2 cells at different time points after serum shock, Actb was chose as the housekeeping genes to normalize the qPCR results. (F) Protein levels at 48 h after transfection with siPer2. (G) Per2 levels in HepG2 cells after serum shock. (H) Urate production in HepG2 cells at 48 h after treatment (*P < 0.05; **P < 0.01).